| 周志国，冯雪.甘蓝水分胁迫诱导基因转录因子研究系统的建立[J].广东农业科学,2014,41(22):122-125 |
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甘蓝水分胁迫诱导基因转录因子研究系统的建立 |
| Establishment of research system for water stress induced gene transcription factors in Brassica oleracea |
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| DOI： |
| 中文关键词: 甘蓝 启动子 荧光素酶 转录因子 |
| 英文关键词: Brassica oleracea promoter luciferase transcription factor |
| 基金项目:河北省科技计划项目（12226306） |
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| 摘要点击次数: 1501 |
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| 中文摘要: |
| 构建启动子荧光素酶载体，利用农杆菌介导法将其导入甘蓝愈伤组织中，对获得的愈伤组织进行抗生素抗性筛选，再利用荧光素酶基因进行分子检测确定阳性转基因甘蓝愈伤组织；使用不同浓度的NaCl对转基因甘蓝愈伤组织进行盐胁迫处理。结果发现，不同浓度NaCl处理组的荧光素酶活性表达均有所提高。并通过网站TFSEARCH 预测，建立了植物转录因子研究模型。结合已有研究结果，利用实时定量PCR 技术检测预测的转录因子mRNA表达量，发现在NaCl刺激下CRP和MADS表达量均升高。 |
| 英文摘要: |
| In this study, the vector of promoter luciferase was built and transfected into kale callus using agrobacterium
mediated method. The luciferase gene was confirmed to be integrated into Brassica oleracea genome with luciferase
detection through preliminary screening with resistance genes. Gradient NaCl was applied in the processing of callus
processing detection. The results showed that in different concentration of NaCl treatments, the expression of luciferase
activity increased. Through the TFSEARCH prediction, the study model of transcription factors in plants was established.
Combined with existing research results, the mRNA expression of CRP and MADS detected by using real-time quantitative
PCR, increased under the stimulation of NaCl. |
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