| 徐自力1，应濠泽1，余佳宁1，祝锦晶1，梁莉佳1，沈广圳1，张丽娜1，杜照奎1,2,3.花生RuBPCase小亚基基因的克隆与序列分析[J].广东农业科学,2014,41(24):122-125 |
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花生RuBPCase小亚基基因的克隆与序列分析 |
| Cloning and expression analysis of RuBPCase smallsubunit gene from Arachis hypogaea L. |
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| DOI： |
| 中文关键词: 花生 核酮糖-1, 5-二磷酸羧化/加氧酶 克隆 序列分析 |
| 英文关键词: Arachis hypogaea Ribulose-1,5-bisphosphate carboxylase/oxygenase(RuBPCase) gene cloning sequence
analysis |
| 基金项目:浙江省教育厅科研项目(Y201223322)；浙江省大
学生科技创新活动新苗人才计划项目(2014R428013) |
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| 中文摘要: |
| 根据已知植物的核酮糖-1,5-二磷酸羧化/加氧酶（RuBPCase）小亚基基因序列设计简并引物，采用RTPCR
技术从花生叶片中克隆得到RuBPCase 小亚基基因，命名为AhrbcS，Genbank 登录号为KF607110，该基因编码
区长度为549 bp，编码182 个氨基酸。序列比对结果表明，AhrbcS 编码蛋白与绿豆、菜豆、大豆、短绒“大豆和烟豆等
具有较高的相似性。 |
| 英文摘要: |
| Degenerate primers were designed according to reported ribulose-1,5-bisphosphate carboxylase/oxygenase
(RuBPCase) gene sequences, the coding sequence of RuBPCase small subunit (AhrbcS), numbered KF607110 was
amplified by RT-PCR from cDNA of Arachis hypogaea L., the sequence of AhrbcS was 549 bp in length which encoded
182 amino acids. Multiple sequence alignment analysis showed that A. hypogaea, Glycine max, Phaseolus vulgaris, G. max,
G. tomentella, and G. tabacina shared high homology. |
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