| 张卫东 王 岚 冯宇梅 廖 力 权永兵 水克娟 徐淼锋 迟远丽 黄永辉.实时荧光PCR 法特异性检测棉花曲叶病毒[J].广东农业科学,2015,42(2):126-130 |
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实时荧光PCR 法特异性检测棉花曲叶病毒 |
| Rapid detection of cotton leaf curl virus by real-time PCR |
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| DOI： |
| 中文关键词: 棉花曲叶病毒（CLCuV） 运动蛋白基因 实时荧光 PCR 技术 |
| 英文关键词: Key words cotton leaf curl virus (CLCuV) movement protein gene real-time fluorescent PCR |
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| 中文摘要: |
| 根据棉花曲叶病毒（Cotton leaf curl virus，CLCuV）运动蛋白基因序列特征，设计并合成了特异性Taqman 探针和检测引物，建立该病毒的实时荧光 PCR 快速检测方法。 结果表明：该方法可以特异性地从 5 个不同 CLCuV 分离物中扩增到荧光信号，而其他 6 种病毒均无扩增。 建立的方法具有快速、准确、灵敏及简便等特点，可为进出境植物检疫和农业安全生产提供可靠的技术支持。 |
| 英文摘要: |
| In this study, special Taqman fluorescent probes and primers were designed based on the sequence ofmovement protein gene, a rapid and effective method of cotton leaf curl virus (CLCuV) detection was established basedon real-time fluorescent PCR. The result showed that the method could amplify specifically fluorescence signal from 5different isolates of CLCuV, while the other 6 viruses had no amplification. The method is not only rapid, accurate,convenient, and sensitive, but also can provide well technical support for Entry-Exit Inspection and Quarantine andagricultural safety. |
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