| 柯 野 伍嘉慧 曾松荣 周 威.一株产蛋白酶菌株的筛选鉴定及酶学性质分析[J].广东农业科学,2015,42(2):137-141 |
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一株产蛋白酶菌株的筛选鉴定及酶学性质分析 |
| Screening and identification of a strain producing proteases and analysis on its enzymatic properties |
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| DOI： |
| 中文关键词: 粘质沙雷菌 鉴定 蛋白酶 酶学性质 |
| 英文关键词: Serratia marcescens identification protease enzymatic properties |
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| 摘要点击次数: 1831 |
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| 中文摘要: |
| 通过测量比较在牛奶平板上产生水解圈的大小，从韶关土壤中分离筛选获得一株产蛋白酶的 ms2菌株。对该菌株的形态结构、生理生化特性以及 16S rDNA 序列的比对分析，鉴定该菌株为粘质沙雷菌（Serratiamarcescens）。 对该菌株进行液体发酵，其发酵液中的蛋白酶最适反应 pH 值为 10.0，表明发酵液中含有碱性蛋白酶；最适反应温度 45益，45益保温处理 100 min 后，酶活性仍保留 70%以上，表明蛋白酶具有较强的热稳定性。 1 mmol/L 的 Mn2+对酶有激活作用，但 1 mmol/L 的 Cu2+和 Fe2+对酶具有明显的抑制作用；EDTA 对蛋白酶也具有明显的抑制作用，说明发酵液中含有金属蛋白酶。 与 Alcalase 碱性蛋白酶相比，发酵液中的蛋白酶对大豆分离蛋白水解能力更强。 在单因素试验中，干酪素和蔗糖分别为氮源和碳源时，发酵液蛋白酶的活性最高。 |
| 英文摘要: |
| A strain producing protease was isolated from soil of Shaoguan city, by determining hydrolytic zone inmilk plate media. The strain was identified as Serratia marcescens, according to morphologic observation,physiological tests, biochemical tests, and 16S rDNA sequence alignment. The fermentation broth of the ms2 straincontained alkaline protease, because the optimum reactive pH of proteases was 10.0. The proteases had maximalactivity at 45, and remained beyond 70% activity for 100 min at 45. The proteases activities were improved byMn2 +, but were inhibited by Cu2 + and Fe2 +. Their activities were strongly inhibited by EDTA, but not by -mercaptoethanol. So the results indicated that the fermentation both had some kind metallo protease. Compared withAlcalase protease, the proteases showed higher hydrolysis activity to soy isolate protein. In single factor tests, whenthe nitrogen sources and the carbon sources individually were casein and sucrose, the fermentation broth had thehighest protease activity. |
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