| 罗 纯， 张青林， 罗正荣.第二代测序技术在植物遗传研究中的应用[J].广东农业科学,2015,42(3):186-192 |
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第二代测序技术在植物遗传研究中的应用 |
| Application of next-generation sequencing technology in plant genetic research |
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| DOI： |
| 中文关键词: 高通量测序 新一代测序 转录组测序 miRNA 测序 |
| 英文关键词: high throughput sequencing next -generation sequencing transcriptome sequencing miRNA
sequencing |
| 基金项目: |
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| 摘要点击次数: 2149 |
| 全文下载次数: 764 |
| 中文摘要: |
| 第二代测序技术，又称“新一代测序技术”，其较 Sanger 测序具有通量高、测序成本低和测序时间
短等特点。 介绍了以 454、Illumina/Solexa 和 SOLiD 为代表的第二代高通量测序技术的基本特性及其在植物全
基因组、转录组和 miRNA 测序等领域的研究进展，并对新一代高通量测序技术的发展趋势进行了展望。 |
| 英文摘要: |
| The research was carried out to study the effects of four kinds of chemical reagents (KNO3, GA3,KMnO4 and NaOH) on seed germination and seedling growth characteristics of Senna alata L. The results indicated that all concentrations of KMnO4 and GA3 promoted the germination and seeding growth of S. alata L., and 0.1g/LGA3 and 4g/L KMnO4 were the most effective reagents. All KNO3 concentrations promoted the germination, but the growthMof seedlings were not affected. The germination process and shoot height were depressed under different concentrations of NaOH, the larger the concentration was, the more severely the seed germination and seedling |
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