| 余海立， 雷生姣， 黄 超.酶法降解与微生物发酵结合处理柑橘皮渣生产高蛋白饲料[J].广东农业科学,2015,42(5):74-78 |
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酶法降解与微生物发酵结合处理柑橘皮渣生产高蛋白饲料 |
| Production of high-protein feed using citrus peel residue by enzymatic degradation combined with microbial fermentation |
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| DOI： |
| 中文关键词: 柑橘皮渣 纤维素酶 微生物发酵 高蛋白饲料 |
| 英文关键词: citrus peel residue cellulose microbial fermentation high-protein feed |
| 基金项目: |
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| 中文摘要: |
| 以柑橘皮渣为原料‘通过超微粉碎技术进行前处理‘采用双酶法降解纤维素与微生物发酵法生产
高蛋白饲料。 以可溶性蛋白质、还原性糖为考察指标‘研究果胶酶与纤维素酶添加量、体系 pH 值、酵母接种量、
发酵时间等因素对发酵终产品的影响‘通过正交试验优化确定最佳生产工艺。 结果表明‘在 50益时‘果胶酶添
加量 0.06 g/100g、纤维素酶添加量 0.02 g/100g、pH 值 5.0 下酶解皮渣 1.0h‘可得还原性糖含量适宜、酵母利用
度高的皮渣液。同时调节该酶解液 pH 值为 4.0‘酵母接种量为 10.0%（V/V）‘35益发酵 5d‘可得可溶性蛋白含量
为 101.9 mg/L 的高蛋白饲料。 |
| 英文摘要: |
| In a bid to product high -protein feed through double enzymatic degradation of cellulose and
microbial fermentation, the citrus peel residue was chosen as the raw material by the superfine grinding technology of
pretreatment. With the soluble protein and reducing sugar as examining index, the main impact factors including
adding amount of pectase and cellulose, system pH, yeast inoculation quantity and cultivate time were investigated.
The technological conditions of fermentation were optimized by orthogonal experiment. The results showed that the
best technological conditions of fermentation were as follows: 0.06 g/100g of pectase and 0.02 g/100g of cellulose at
50益in pH 5.0 for 1 h, the citrus peel residue with efficacy reducing sugar and yeast exploitation degree was
obtained. Then the citrus peel residue was cooled to 35益, inoculation quantity 10.0% (V/V), pH 4.0 and cultivate
time 5 d were optimal for the increasing of protein content in fermentation production, the yield of soluble protein
was 101.9 mg/L. |
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