潘建刚, 常 亚, 高国日,等.无机解磷菌的分离鉴定及解磷条件优化[J].广东农业科学,2015,42(6):51-55 |
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无机解磷菌的分离鉴定及解磷条件优化 |
Isolation, identification of phosphate-solubilizingmicroorganism (PSM) and optimizationof solubilizing phosphate |
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DOI: |
中文关键词: 解磷菌 解磷能力 条件优化 16S rRNA 系统发育分析 |
英文关键词: phosphate-solubilizing microorganism (PSM) solubilizing phosphate capability optimization 16S
rRNA phylogenetic analysis |
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中文摘要: |
解磷菌对于提高土壤磷元素的有效利用具有促进作用,而且对于土壤面源污染的防治也具有重
要意义。 通过对包头地区草坪土壤微生物的筛选,获得 3 株具有稳定解磷能力的解磷菌 P1、P2 和 P3,其中 P1
的解磷效果最佳;复合菌群组合试验结果表明,不同组合的解磷能力依次为 P1:P3跃P1:P2跃P1:P2:P3跃P2:P3,证实
P1 具有高效的解磷能力;分子鉴定结果表明,P1 是伯克霍尔德氏菌(Burkholderia sp.),说明该菌具有较大的应
用价值。 |
英文摘要: |
Phosphate-solubilizing microorganism (PSM) can promote the effective utilization of soil phosphorus,
and play an important role in the control of non-point source pollution of soil. Three PSMs (P1, P2 and P3) with
stable phosphate -solubilizing ability were isolated from the turfgrass soil in Baotou, in which the solubilizing
phosphate ability of P1 was higher than that of the other two strains. The results about a series experiment on
composite bacteria system showed the order of phosphate-solubilizing ability was P1:P3 P1:P2 P1:P2:P3跃P2:P3,
which confirmed that P1 had high ability of dissolving phosphorus. The molecular identification showed that P1
belonged to Burkholderia sp., it had great application value in practice. |
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