| 任 禛， 黄鹤平， 陈泽斌，等.黄瓜根际土壤及根系的AMF菌群多样性研究[J].广东农业科学,2015,42(6):105-110 |
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黄瓜根际土壤及根系的AMF菌群多样性研究 |
| Diversity of arbuscular mycorrhizal fungi communityin the rhizosphere soil and roots of cucumber |
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| DOI： |
| 中文关键词: 丛枝菌根真菌 18S rRNA 基因 巢式 PCR 系统发育树分析 |
| 英文关键词: arbuscular mycorrhizal fungi 18S rRNA gene Nested-PCR phylogenetic tree analysis |
| 基金项目: |
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| 中文摘要: |
| 从昆明学：种植基地随机采集黄瓜根系及根际土壤样品，采用巢式 PCR 技术分别扩增菌根及土
壤丛枝菌根真菌（AMF）部分 18S rRNA 基因区域，利用该产物构建 AMF 克隆文库，并结合 ARDRA 筛选、DNA
测序和系统发育树分析对黄瓜 AMF 菌群组成进行研究。 结果表明： 根际土壤检测到 7 种 AMF： 摩西球囊霉
（Glomus mosseae）、根内球囊霉（G. intraradices）、地表球囊霉（G. versiforme）以及其他 4 种未知的 Glomus 属类
型，而根系除发现了 G. mosseae、G. intraradices 和 G. versiforme 外，仅检测到了其他 2 种未知 Glomus 属种类。 |
| 英文摘要: |
| Cucumber roots and rhizosphere soil samples were collected randomly from Kunming College planting
base. The partial 18S rRNA gene of two samples were amplified by Nested-PCR. These DNA fragments were used to
construct AMF clone libraries. AMF community composition were studied by ARDRA screening, DNA sequencing
and phylogenetic tree analysis. The results showed that seven species were obtained in rhizosphere soil. They were
Glomus mosseae, G. intraradices, G. versiforme and other four unknown Glomus types. Except for G. mosseae, G.
intraradices and G. versiforme, only two unknown Glomus species were detected in roots. |
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