| 陈雪香，刘晓娟，周丽萍，等.山莓叶乙醇提取物与抗生素的协同抑菌效果研究[J].广东农业科学,2015,42(7):-76 |
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山莓叶乙醇提取物与抗生素的协同抑菌效果研究 |
| Synergistic antimicrobial effects of ethanol extracts of Rubus corchorifolius L.f leaves and antibiotics |
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| DOI： |
| 中文关键词: ：山莓叶 抑菌效果 抗生素 协同作用 |
| 英文关键词: Rubus corchorifolius L.f. leaves antimicrobial activity antibiotic synergistic effect |
| 基金项目: |
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| 摘要点击次数: 1747 |
| 全文下载次数: 875 |
| 中文摘要: |
| ：采用K-B 纸片法检测山莓叶乙醇提取物（RE）、抗生素对 3种致病菌（大肠杆菌、痢疾志贺氏菌、
沙门氏菌）的最低抑菌浓度（MIC），再将 RE 与抗生素联合使用，用棋盘法与K-B 纸片法相结合进行抑菌效果
评价。结果显示：对于大肠杆菌、痢疾志贺氏菌、沙门氏菌，RE的MIC分别为6.250、6.250、3.125…mg/mL；RE与
金霉素协同使用的分级抑菌浓度（FIC）最小值分别为 0.313、0.375 和 0.313，协同抑菌效果最好的配比分别为
1/8MIC1 ∶1/2MIC2、1/4MIC1 ∶1/2MIC2 和1/8MIC1 ∶1/2MIC2；RE与净球散协同的FIC 最小值分别为 0.375、0.313和
0.375，协同抑菌效果最好的配比分别为1/4MIC1 ∶1/2MIC2、1/8MIC1 ∶1/2MIC2 和1/4MIC1 ∶1/2MIC2。大肠杆菌、痢疾志
贺氏菌和沙门氏菌山莓叶乙醇提取物的浓度控制在1/4~1/8MIC1，金霉素和净球散浓度均为1/2MIC2，均有较强的
协同抑菌效果。 |
| 英文摘要: |
| The minimum inhibitory concentration（MIC）of RE and antibiotics on Escherichia coli，Shigella dysenteroae，and Salmonella were measured by Kirby-Bauer text（K-B）. Then Rubus corchorifolius L.f leaves（RE） was used to combine with antibiotics. Fractional inhibitory concentration（FIC） was measured by checkerboard microevolutio and K-B method. The results showed that E. coli，S. dysenteroae and Salmonella were used as microbial strains to test the antibacterial activity，the MICs of RE were 6.250 mg/mL，6.250 mg/mL，3.125 mg/mL，respectively. The minimum FICs of combined use of RE and aureomycin were 0.313，0.375，0.313，and the best ratios were 1/8MIC1∶1/2MIC2， 1/4MIC1∶1/2MIC2，1/8MIC1∶1/2MIC2，respectively. The minimum FICs of combined use of RE and Jingqiusan were 0.375， 0.313，0.375，and the best ratios were respectively. When theconcentration of RE was 1/4~1/8MIC1，and the concentrations of aureomycin and Jingqiusan were 1/2MIC2，the synergistic antimicrobial effects on E. coli，S. dysenteroae and Salmonella were strong. |
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