叶健军,林洁荣,焦文静,等.福建省狼尾草属种质资源RAPD鉴定分析[J].广东农业科学,2015,42(7):-132 |
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福建省狼尾草属种质资源RAPD鉴定分析 |
RAPD analysis of genetic diversity of Penniseum germplasm resources in Fujian |
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DOI: |
中文关键词: :狼尾草 遗传多样性 RAPD分析 |
英文关键词: Pennisetum Rich genetic diversity RAPD analysis |
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中文摘要: |
采用RAPD标记分析了18…份狼尾草属种质的遗传多样性及亲缘关系,结果表明:19…条引物在18…份
材料中共扩增出207…条条带,其中176…条具有多态性,多态比率85%,各引物扩增条带数为6~15…条不等,遗传距
离的变幅为0.0819~1.011,平均为0.4713;遗传相似系数变幅为0.4907~0.9352,平均为0.6871。聚类结果表明:遗
传距离以0.7为值大致可以分为4…类:巨菌草、热研 4…号王草、桂草1…号王草、桂牧1…号杂交象草、紫象草聚为一
类;杂交狼尾草和细茎象草分别单独聚为一类;剩余的品种聚为第四类。 |
英文摘要: |
The genetic diversity and relationships of 18 different Penniseum Rich germplasms were analyzed using
Random Amplified Polymorphic DNA(RAPD)markers in order to provide essential information for the development and
utilization of Pennisetum germplasm resources. RAPD analysis demonstrated that 19 random primers produced 207 loci,
among which 176 were polymorphic loci,the percentage of polymorphic loci was 85%,and each primer produced 6-15
belts. Genetic distance changed from 0.0819 to 1.011,averaged 0.4713;Genetic similarity changed from 0.4907 to 0.9352,
averaged 0.6871. According to clustering analysis,we classified the 18 materials into four groups by 0.7,Giant Juncao,
P. purpureum×P. americanurn. cv. Reyan No.4,P. purpureum×P. americanurn. cv. Guicao No.1,(P. americanum×
P. purpureum)×P. durpureum cv.Guimu No.1,P. purpureum Schum cv. Purple clustered together into one
group;P. americanum×P. purpureum and P. purpureum Schum cv. Xijing each formed its own category,while the rest
formed one category. |
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