| 唐 丽，李 菁，龙 华，等.不同生长龄铁皮石斛茎与叶中总多糖、 总生物碱及总黄酮含量的差异…[J].广东农业科学,2015,42(8):17-21 |
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不同生长龄铁皮石斛茎与叶中总多糖、 总生物碱及总黄酮含量的差异… |
| Content differences of total polysaccharides，total alkaloidsand total flavonoids from stems and leaves of Dendrobiumcandidum at different growth ages |
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| DOI： |
| 中文关键词: 铁皮石斛 生长龄 总多糖 总生物碱 总黄酮 |
| 英文关键词: Dendrobium candidum growth age total polysaccharides total alkaloids total flavonoids. |
| 基金项目: |
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| 摘要点击次数: 1542 |
| 全文下载次数: 1037 |
| 中文摘要: |
| 采用苯酚硫酸法、酸性染料比色法以及硝酸铝比色法分别测定并比较不同生长龄铁皮石斛茎与叶
中总多糖、总生物碱及总黄酮的含量差异，为铁皮石斛资源的充分合理利用提供科学依据。结果表明，不同生长
龄铁皮石斛茎与叶中总多糖、总生物碱、总黄酮的含量均有显著差异，其中总多糖主要集中于茎，总生物碱和总
黄酮主要集中于叶；总多糖含量以2 年龄茎最高，为 35.32%，总生物碱含量以1年龄叶最高，为 0.036%，总黄
酮含量以半年生叶最佳，为 0.104%；总多糖含量随着生长龄的增加而逐渐减少；3 年龄以内的总生物碱和总黄
酮含量明显较高，且不同龄之间含量差异较小，而当植株生长至 4、5 年龄时，其总生物碱及总黄酮的含量明显
较低。铁皮石斛茎与叶中各含有较高的不同有效成分，茎和叶均可作为药用资源加以充分利用；为获取最佳效
益，综合考虑生长周期、生物量及药用成分含量等因素，人工栽培的铁皮石斛以3年龄内采收为宜，不宜栽种 4
年以上。 |
| 英文摘要: |
| To provide scientific basis for making full use of Dendrobium candidum resources，contentdifferences of total polysaccharides，total alkaloids and total flavonoids from stems and leaves of D. candidumat different growth ages were determined using phenol vitriolic method，acid-dye and Al（NO3）3 colorimetricmethod. Results showed that there were significant dif ferences in the contents of total polysaccharides，total alkaloids and total flavonoids from stems and leaves of D. candidum at dif ferent growth ages. Totalpolysaccharides mainly concentrated in stems，but total alkaloids and total flavonoids mainly concentratedin leaves. The highest content of total polysaccharides was about 35.32% for stem of two-year D. candidum，but the highest content of total alkaloids was about 0.036% for one-year leaf，and the highest content oftotal flavonoids was about 0.104% for half-year leaf. Content of total polysaccharides gradually decreasedwith growth age，and contents of total alkaloids and total f lavonoids of D. candidum in three years weresignificantly high. When the growth age reached four or five years，the contents of total alkaloids and totalf lavonoids were signif icantly low. Each stem andlea f of D. candidum contained dif f er ent activein g r ed ien t s，so b o t h o f t h em co u l d be used a smedicinal resources. In order to obtain the bestbenefit and considering the growth cycle，biomassand medicinal ingredients and so on，the appropriatetime for artificial-cultivation of D. candidum wasthree years and not more than 4 years. |
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