| 王晓林，杜晓宇，钟方丽，等.刺玫叶总皂苷富集工艺条件优化[J].广东农业科学,2015,42(8):72-76 |
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刺玫叶总皂苷富集工艺条件优化 |
| Optimization of enrichment technology of total saponinsin leaves of Rosa davurica Pall. with macroporous resin |
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| DOI： |
| 中文关键词: 刺玫叶 大孔树脂 总皂苷 富集 |
| 英文关键词: leaves of Rosa davurica Pall. macroporous absorption resin total saponins enrichment |
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| 摘要点击次数: 1788 |
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| 中文摘要: |
| 以吸附率和解吸率为考查指标，采用静态吸附 - 解吸、动态吸附 - 解吸试验，确定了D-101型大
孔树脂富集刺玫叶总皂苷的工艺条件。结果发现，最佳富集条件为上柱液浓度为原提取液稀释 5 倍、吸附速
率为 2…BV/h、上柱液 pH 值为 7～9，解吸液为 95%乙醇、洗脱速率为 2…BV/h、洗脱液用量为 7…BV。在此条件
下，其平均吸附率为81.33%，平均解吸率为86.31%，纯化后刺玫叶干浸膏中总皂苷含量由原来的16.32% 提高
到 48.13%，树脂富集倍数约为3 倍。结果表明，D-101型大孔树脂对刺玫叶总皂苷的富集具有一定的效果。 |
| 英文摘要: |
| To investigate the enrichment technology of total saponins（ TS） in leaves of Rosa davurica Pall.with macroporous resin，by both static and dynamic absorption-desorption experiments，the optimum purificationconditions with D-101 macroporous resin were determined using absorption and desorption ratio of TS asevaluating indicators. The results showed that the optinal enrichment canditions were as follows：the TS contentof the sample，adsorption rate，pH value of sample，content of ethanol eluant，desorption rate and volume ofeluant were one-fifth content of the raw extracts solution，2 BV/h，7 to 9，70%，2 BV/h，and 7 BV respectively.The average absorption and desorption rates were 81.33% and 86.31% under the optimum conditions. Afterpurification，the TS content in extracts was from 16.32% to 48.13%，which offered 3 times of enrichment withresins. The results indicated that purification of TS in leaves of R. davurica Pall. with D-101 macroporous resinwas effective. |
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