| 于红亮，李新辉，吴 垠，等.几种不同食性鱼EST 及α-AMY同工酶特性研究[J].广东农业科学,2015,42(8):83-88 |
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几种不同食性鱼EST 及α-AMY同工酶特性研究 |
| Characteristics of EST and α-AMY isozymesin fishes with different food structures |
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| DOI： |
| 中文关键词: EST α-AMY 同工酶 食性 电泳 |
| 英文关键词: EST α-AMY isozyme food structure electrophoresis |
| 基金项目: |
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| 中文摘要: |
| 采用不连续聚丙烯酰胺垂直板凝胶电泳的方法，对广东鲂（Megalobrama terminalis）、 （Hemiculter
leucisculus）、鲮（Cirrhina cuvier）、草鱼（Ctenopharyngodon idellus）、青鱼（Mylopharyngodon piceus）、鳜（Siniperca
chuatsi）6 种不同食性鱼的肝脏、肌肉、心脏、眼、鳃、肾等6 种新鲜组织的酯酶（EST）及α-淀粉酶（α-AMY）同
工酶进行了比较研究。结果表明这6 种不同食性鱼6 种组织中均存在EST和α-AMY同工酶的分布，他们具有
明显的组织特异性；在这些鱼中，EST同工酶的种类和表达程度较α-AMY同工酶都占优势；肝脏和肾脏中EST
和α-AMY 同工酶的活性高于其他组织；EST 和α-AMY 同工酶在不同食性鱼中表现出明显的差异。通过对
EST和α-AMY同工酶的特性分析，可了解并研究鱼类当前的食性状态，进一步掌握鱼类栖息环境的营养源与生
态状况。 |
| 英文摘要: |
| By means of poyacrylamid gel electrophoresis，two isozymes（EST，α-AMY）in liver，muscle，heart，eye，gill，kidney of six kinds of adult fish including Megalobrama terminalis，Hemiculter leucisculus，Cirrhina cuvier，Ctenopharyngodon idellus，Mylopharyngodon piceus，Siniperca chuatsi were studied. The results showed that the ESTand α-AMY isozymes were very complicated and all the isozyme phenotypes exhibited tissue-specificity. The species andexpression degree of EST isozyme was higher than that of α-AMY isozyme in these fishes. The activities of EST isozymeand α-AMY isozyme in liver and kidney were higher than those in other organs. There exited difference in differentfeeding fishes，so analysis on the characteristic of EST and α-AMY isozymes could reveal the current status of feedinghabitat，know the nutrition sources and ecological condition of fish habitats. |
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