| 王 丽，张剑亮，王继华，等.甘蔗品种RSD 感病性检测及RSD 病原菌分离培养[J].广东农业科学,2015,42(9):62-67 |
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甘蔗品种RSD 感病性检测及RSD 病原菌分离培养 |
| Identification of ratoon stunting disease（RSD）of differentsugarcane varieties and isolation of RSD pathogen |
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| DOI： |
| 中文关键词: 甘蔗宿根矮化病（RSD） 感病率 分离培养 |
| 英文关键词: sugarcane ratoon stunting disease（RSD） infective rate isolation |
| 基金项目: |
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| 中文摘要: |
| 为了解不同甘蔗品种的甘蔗宿根矮化病（RSD）感病情况，采用PCR 检测方法，对13 个甘蔗品种进
行RSD 感病率检测。结果显示，在所检测的13 个甘蔗品种中，不同品种感病率有所差异，其中所检测的2 个CP
系列感病率最低。同时，利用MSC 培养基从感染RSD 的甘蔗蔗汁中分离培养到一种生长缓慢、菌落颜色为乳白
色的杆状细菌。根据菌落形态、显微镜观察，以及特异引物和16S rDNA 扩增多种方法鉴定，最终确认所分离到的
杆状细菌即为甘蔗宿根矮化病病原菌（Leifsonia xyli subsp. xyli）。 |
| 英文摘要: |
| In order to understand the inf ective rate of ratoon stunting disease（RSD）of dif f erentsugarcane varieties，13 sugarcane varieties were identified by PCR. The results showed that the infectiverates of different sugarcane varieties were different，and two CP sugarcane varieties had the lowest infectiverates. And a kind of coryneform bacterium with slow growth and milky white colony was isolated from RSDinfected sugarcane juice by MSC culture. By colonial morphology，microscopic examination，specific primersand identification of 16S rDNA，the coryneform bacterium was identified as Lei f sonia xyli subsp. xyli，thepathogen of sugarcane RSD. |
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