| 林海丹，谢守新，王 岚，等.超高效液相色谱串联质谱法测定果蔬中13 种植物生长调节剂残留量[J].广东农业科学,2015,42(9):82-87 |
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超高效液相色谱串联质谱法测定果蔬中13 种植物生长调节剂残留量 |
| Determination of 13 plant growth regulator residues in fruitsand vegetables using ultra performance liquidchromatography tandem mass spectrometry |
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| DOI： |
| 中文关键词: 高效液相色谱串联质谱 果蔬 植物生长调节剂 |
| 英文关键词: liquid chromatography tandem mass spectrometry fruits and vegetables plant growth regulator |
| 基金项目: |
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| 摘要点击次数: 1900 |
| 全文下载次数: 794 |
| 中文摘要: |
| 建立了超高效液相色谱串联质谱法同时测定果蔬13 种植物生长调节剂残留量的方法。样品经甲醇
水提取，用Shim-pack XR C18 色谱柱（75 mm×2.0 mm，1.6 μm），以乙腈-0.1% 乙酸溶液作为流动相梯度洗脱，采
用电喷雾离子化，正、负离子扫描，多反应选择离子监测，基质匹配外标法峰面积定量。矮壮素、多效唑、烯效唑、
噻苯隆、赤霉素、2—奈乙酸、氯吡脲、2，4—D、调果酸、4—氯苯氧乙酸、2，4，5—三氯苯氧乙酸、6—BA 和氯苯胺灵在
质量浓度1~20 μg/L 范围内线性良好，相关系数 r 均大于0.99，样品在10、20、50 μg/kg 等3 个添加水平的平均回
收率为81.2%~106%，变异系数为2.13%~11.8%（n=6），方法的定量限为0.022~16 μg/kg。该方法简便、特异性好，
适用于果蔬中13 种植物生长调节剂的检测。 |
| 英文摘要: |
| To develop a ultra performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS）method for the determination of 13 plant growth regulator residues in fruits and vegetables. The sample wasextracted with methanol–H2O and the chromatographic separation was performed on the Shim-pack XR C18 column（75 mm × 2.0 mm，1.6 μm），using acetonitrile-0.1% acetic acid as the mobile phase by gradient elution. Themethod applied electrospray ionization in positive and negative multiple reaction monitoring mode and quantifiedwith peak area with matrix-matched external standard method. A good linearity of Chlormequat chloride，Paclobutrazol，Uniconazole，thidiazuron，gibberellic acid 2-naphthaleneacetic acid，Forchlorfenuron，2，4-D，Cloprop，4-Chloroph-enoxyacetic acid，2，4，5-Trichlorophenoxyacetic acid，6-Benzylaminopurine and isopropylN-（3-chlorophenyl）carbamate were in the concentration range of 1.0-20 μg/L with a correlation coefficient above0.99. The average recoveries from three different were with the range of 81.2% to 106% at spiked levels of 10，20，50 μg/kg，and the relative standard deviations were between 2.13% to 11.8%（n=6）. The limit of quantification wasin the range of 0.022~16 μg/kg. This method is simple，selectivity and suitable for determination of 13 residues infruits and vegetables. |
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