王继华,马宗艺,罗梦晓,等.小水榕组织培养中的细菌污染及鉴定[J].广东农业科学,2015,42(13):60-64 |
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小水榕组织培养中的细菌污染及鉴定 |
Contamination and identification of bacteriain tissue cultures of Anubias barteri |
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DOI: |
中文关键词: 植物内生菌 小水榕 组织培养 16S rDNA |
英文关键词: plant endophytic bacteria Anubias barteri tissue culture 16S rDNA |
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中文摘要: |
对小水榕组织培养过程中的污染细菌进行分离鉴定,结果表明:分离到的13 株污染细菌分别属于
4 个类群(Alphaproteobacteria、Betaproteobacteria、Gammaproteobacteria、Actinobacteria)的11 个属(Paracoccus、
Sphingomonas、Agrobacterium、Delftia、Roseateles、Cupriavidus、Herbaspirillum、Stenotrophomonas、Acinetobacter、
Microbacterium、Mycobacterium),其中Betaproteobacteria 为优势种群,占总数的38.5%;所有菌株和其同源菌株的
相似性均在98% 以上。 |
英文摘要: |
Bacteria in tissue culture of Anubias barteri were isolated and identified in this study. The results
showed that 13 isolates belonged to 11 genus(Paracoccus,Sphingomonas,Agrobacterium,Del ftia,Roseateles,
Cupriavidus,Herbaspirillum,Stenotrophomonas,Acinetobacter,Microbacterium,Mycobacterium)of 4 groups
(Alphaproteobacteria,Betaproteobacteria,Gammaproteobacteria,Actinobacteria). 38.5% of all isolates were
Betaproteobacteria that were dominant species. The result was beneficial for preventing the contamination of
production in the future |
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