| 丁西朋，罗小燕，邵辰光，等.Genomic-SSR 和EST-SSR在柱花草种间的遗传差异[J].广东农业科学,2015,42(14):105-113 |
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Genomic-SSR 和EST-SSR在柱花草种间的遗传差异 |
| Comparative analysis of genetic diversity revealedby genomic-SSR and EST-SSR markersin interspecies of Stylosanthes |
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| DOI： |
| 中文关键词: 柱花草 EST-SSR Genomic-SSR 遗传差异 |
| 英文关键词: Stylosanthes spp. EST-SSR Genomic-SSR genetic differences |
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| 中文摘要: |
| 为了探讨genomic-SSR 与EST-SSR 标记在柱花草种间的遗传差异，利用两种SSR 标记技术，对来
自不同种的12 份柱花草种质进行了遗传分析，并对两种不同标记进行比较。结果表明：EST-SSR 标记相比
genomic-SSR 在柱花草不同种间具有更好的通用性，EST-SSR 检测到的等位基因数和PIC 指数平均值分别是
4.6 和0.610，genomic-SSR 检测到的等位基因数和PIC 指数平均值分别是5.6 和0.702。聚类结果表明，
genomic-SSR 和EST-SSR 均能有效鉴别所有供试柱花草种质。 |
| 英文摘要: |
| To explore the genetic differences between genomic -SSR and EST -SSR in different species of
Stylosanthes, the genetic diversity of 12 accessions from different Stylosanthes species was analyzed by two types of
SSR markers. Results showed that, compared with genomic-SSR, EST-SSR markers were superior in versatility for
different species of Stylosanthes. The average of number of alleles and the polymorphic information content (PIC)
value detected by EST -SSR was 4.6 and 0.610, respectively. The average of number of alleles and PIC value
detected by genomic-SSR was 5.6 and 0.702, respectively. Cluster analysis indicated that two SSR markers could
effectively identify all Stylosanthes accessions |
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