| 毛心莹1，苏 生2，曹红旗3，沈 玲4，韩永芬2，李 䶮1.多花木蓝抑菌活性及其化学成分研究[J].广东农业科学,2015,42(15):48-52 |
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多花木蓝抑菌活性及其化学成分研究 |
| Antimicrobial activities and chemical constituentsof Indigofera amblyantha Crai |
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| DOI： |
| 中文关键词: 多花木蓝 抑菌活性 籽石油醚提取物 化学成分 |
| 英文关键词: Indigofera amblyantha Craib antimicrobial activities petroleum ether extracts of
seed chemical constituents |
| 基金项目:贵州省自然科学基金（黔科合J 字［2013］
2154 号）；西安工程大学博士科研启动项目（BS1310） |
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| 中文摘要: |
| 采用超声波提取法，选取石油醚、氯仿、乙酸乙酯、丙酮、甲醇为提取溶剂，得到豆科植物多花木
蓝根、茎、叶、籽不同溶剂的提取物。采用抑制菌丝生长法，选取稻瘟病菌、辣椒枯萎病菌、小麦赤霉病菌、油
菜菌核病菌、梨黑星病菌5 种植物病原真菌为测试菌，测试多花木蓝各提取物抑制真菌的活性。结果显示，多
花木蓝茎、叶各溶剂提取物均没有显示抑菌活性，根、籽提取物有一定的抑菌活性，籽提取物的活性强于根部
提取物，根部提取物随着提取溶剂极性的增加活性亦增加，其甲醇的生物活性最好，抑制率分别为65.31%、
69.09%、72.50%、61.06%、64.95%；与此不同，籽石油醚提取物的抑制率高于其他溶剂提取物，其抑制率分别
为71.43%、72.73%、79.04%、74.34%、74.02%。采用牛津杯法，选取大肠杆菌、青枯菌为测试菌，测试多花木
蓝各提取物抑制细菌的作用，结果显示，根、茎、叶不同溶剂提取物均没有抑制细菌的活性，籽石油醚提取物
显示出一定的抑菌活性，其对青枯菌的抑菌圈直径平均值为1.45 cm，对大肠杆菌的抑菌圈直径平均值为1.41
cm，但籽其他溶剂提取物亦没有显示出抑菌活性。GC-MS 分析籽石油醚提取物的化学成分，结果共鉴定出其
中17 种成分，占精油色谱峰总面积的99.065%，其中亚油酸含量最高，达到37.569%。 |
| 英文摘要: |
| By associated ultrasonic extracting method，the different parts of Indigofera amblyantha Craib extracts
were prepared with petroleum，chloroform，ethyl acetate，acetone and methanol，respectively. The fungistatic activities
were detected against fungi including Magnaporthe grisea（Hebert）Barr，Fusariumoxysporum f. sp. Vasinfectum
（Atk.）Synder et Hansen，Fusarium graminearum Schw.，Sclerotinia sclerotiorum（Lib.）de Bary，Venturia pyrina.
The results showed that the root and seed extracts had medium bioactivities，but the stem and leaf extracts had no
bioactivity. The antifungal activity of root extracts was lower than that of seed extracts. Moreover，antifungal activity of
root extracts increased with the polarity of extraction solvents increasing. The antifungal activity of methanol extracts of
root was the strongest，the antifungal activities on above fungi were 65.31%，69.09%，72.50%，61.06% and 64.95%，
respectively. However，petroleum ether extracts showed the strongest bioactivity in all seed extracts，and the antifungal
activities on above fungi were 71.43%，72.73%，79.04%，74.34%，74.02%，respectively. The antibacterial activities of
extracts were also assessed against bacteria（Ralstonia solanacearum，Escherichia coli）using disc method. The results
showed that petroleum ether extracts of seed had medium activity，the means of inhibition zones of the above two bacteria
were 1.45 cm and 1.41 cm，respectively. No others
showed activities. The result of Gas Chromatography-
Mass Spectrometer（GC-MS）analysis indicated that 17
compounds，according for 99.065% of total，were present
in the petroleum ether extracts of seed，and linoleic acid
（37.569%）was the major chemical constituent |
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