| 朱 辉1，覃伟权1，付登强1，戚志强2.海南油茶炭疽病病原鉴定及生物学特性研究[J].广东农业科学,2015,42(16):55-54 |
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海南油茶炭疽病病原鉴定及生物学特性研究 |
| Identification and biological characteristics of thepathogen of oil tea anthracnose in Hainan |
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| DOI： |
| 中文关键词: 油茶炭疽病 病原鉴定 rDNA-ITS 序列 β- 微管蛋白基因 生物学特性 |
| 英文关键词: oil tea anthracnose pathogen identification rDNA internal transcribed spacer sequence TUB2
gene biological characteristic |
| 基金项目:海南省自然科学基金（314147）；中国
热带农业科学院院本级基本科研业务费专项（163003
2015006） |
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| 中文摘要: |
| 为了明确海南油茶炭疽病的病原，对病原菌的形态学特征、致病性、分子序列和生物学特性进行
研究。结果表明，病原菌分生孢子长卵圆形或圆柱形，大小为13.0~20.3 μm×3.7~6.2 μm；基于转录间隔区和
β- 微管蛋白基因部分序列的系统进化分析结果表明，病原菌与胶孢炭疽菌标准菌株处在进化树的同一分支，
其自展支持率达100%；碳源、氮源、温度和pH 值对病原菌生长的影响差异均显著，病原菌生长的最佳碳、氮
源分别为海藻糖和蛋白胨，最适生长温度和pH 值分别为28℃和6。据此将海南油茶炭疽病的病原菌鉴定为胶
孢炭疽菌Colletotrichum gloeosporioides（Penz.）Sacc.。 |
| 英文摘要: |
| In order to identify the pathogen of oil tea anthracnose in Hainan，morphological characteristics，
pathogenicity test，analysis of molecular sequences and biological characteristics were studied. The results showed that
Conidia were oval or cylindrical，measured 13.0-20.3 μm×3.7-6.2 μm. The results based on the phylogenetic analysis
of the rDNA-ITS and TUB2 gene showed that the pathogen was in the same branch with Colletotrichum gloeosporioides
reference isolates in the phylogenetic tree，whose bootstrap value was 100%. Effects of carbon source，nitrogen source，
temperature and pH on pathogen growth were significantly different. The optimal carbon source and nitrogen source for
pathogen growth were trehalose and peptone respectively. The optimal temperature and pH value for pathogen growth
were 28℃ and 6 respectively. Based on above results，the pathogen of oil tea anthracnose in Hainan was identified as
Colletotrichum gloeosporioides（Penz.）Sacc. |
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