| 龚 丽，陈兰明.弧菌基因组中整合性接合元件
（ICEs）的预测分析[J].广东农业科学,2015,42(16):96-95 |
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弧菌基因组中整合性接合元件
（ICEs）的预测分析 |
| Predictive analysis of integrative and conjugative elements（ICEs）in completely sequenced Vibrio genomes |
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| DOI： |
| 中文关键词: 弧菌 整合性接合元件 全基因组 |
| 英文关键词: Vibrio integrative and conjugative elements complete genome |
| 基金项目:上海市教育委员会项目（B-9500-10-0004）
作者简介：龚丽（1989-），女，在读硕士生，E-mail：
emo_jun@163.com |
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| 中文摘要: |
| 整合性接合元件（ICEs）是一种广泛存在于细菌中可以自主移动的遗传元件。ICEs 通过水平移动
整合到弧菌的染色体上，所携带的新基因有助于宿主在特定生存环境下获得生长优势。为考察ICEs 在弧菌生
长中发挥的作用，采用PrfC 位点与核心基因相关的特征蛋白，进行了26 株弧菌SXT/R391ICEs 的预测。结果表
明，在全测序的26 株弧菌中，只有3 株霍乱弧菌的较大染色体上存在SXT/R391 ICEs，对这3 株霍乱弧菌上SXT/
R391 ICEs 的抗性基因和热点区域进行比较分析，结果表明，3 株霍乱弧菌的SXT/R391 ICE 的抗性基因和热点区
域虽然都拥有类似的骨架结构，但从获取基因的得失情况可以判断，这3 株菌SXT/R391 ICE 的进化优先关系。 |
| 英文摘要: |
| Integrative and conjugative elements（ICEs）are selftransmissible mobile genetic elements that
mediate horizontal gene transfer in bacteria. ICEs and related elements can constitute a large proportion of bacterial
chromosomes，and bestow a wide range of phenotypes upon their host with carried gene cassettes. To study the role
of SXT/R391 ICEs in the growth of Vibrio， SXT/R391 ICEs were identified in the 26 completely sequenced Vibrio
genome using PrfC and their characteristic modules. The results showed that only SXT/R391 ICEs were found from 3
V. cholerae，and we comparatively analyzed the resistance genes and hotspots among 3 SXT/R391 ICEs. The region in
resistance genes and hotspots had similar structure in 3 SXT/R391 ICEs，it could indicate the precedence relation in 3
SXT/R391 ICEs based on the genes which lost or gained |
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