文章摘要
范兰芬, 钟杨生, 陈芳艳, 严会超, 林健荣.蚕卵经盐酸浸渍后的卵壳蛋白变化分析[J].广东农业科学,2015,42(17):108-112
查看全文    HTML 蚕卵经盐酸浸渍后的卵壳蛋白变化分析
Analysis on eggshell protein changes aftersilkworm eggs soaked by HCl
  
DOI:
中文关键词: 盐酸  浸渍蚕卵  卵壳蛋白  变化
英文关键词: hydrochloric acid  soaking eggs  eggshell protein  chang
基金项目:国家自然科学基金(31272368) ; 国家现 代农业产业技术体系建设专项(CARS-22) ; 广东省自然 科学基金(S2011010001893) ; 国家公益性行业(农业) 科研专项(201403064)
作者单位
范兰芬, 钟杨生, 陈芳艳, 严会超, 林健荣 华南农业大学动物科学学院 广东 广州 510642 
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中文摘要:
      对 932 品种在卵期发育和幼虫期饲养阶段实施温湿环境调控, 让其产下滞育卵和非滞育卵。 滞育 卵经即时浸酸处理后, 用扫描电镜观察卵壳的变化, 可见卵壳表面比对照出现较多的裂痕。 通过 SDS-PAGE 电泳技术, 比较滞育卵、 滞育卵经即时浸酸处理、 非滞育卵的卵蛋白变化, 发现滞育卵经浸酸处理后, 20 ku 以 下的小分子量蛋白明显减少。 利用 shotgun 技术对此类小分子量蛋白组分进行鉴定分析, 发现在滞育卵和非滞 育卵中都存在 ErB.1、 ErB.2、 ErB.3、 Hc-A 和 Hc-B 卵壳蛋白, 在经即时浸酸处理过的滞育卵中却未发现, 由此 推测蚕卵经浸酸处理后会引起这 5 种卵壳蛋白的降解。
英文摘要:
      Diapause and non-diapause eggs were laid by the temperature and humidity regulation in the egg and larvae feeding stage of 932 variety. Diapause eggs by HCl treatment were observed more visible eggshell cracks than the control by scanning electron microscope. The total proteins of diapause eggs, diapause eggs by HCl treatment and non- diapause eggs were compared using SDS-PAGE electrophoresis. Small molecular proteins below 20 ku decreased in the proteins of diapause eggs by HCl treatment. The small molecular proteins were identified by shotgun analysis. The ErB.1, ErB.2, ErB.3, Hc-A and Hc-B eggshell proteins were detected in the diapause and non-diapause eggs, but were not detected in the diapause eggs by HCl treatment. It is speculated that the diapause eggs by HCl treatment may cause the degradation of the five eggshell proteins.
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