任 燕, 孙承文, 石存斌, 潘厚军, 陶家发, 吴淑勤.嗜水气单胞菌毒力因子检测及不同毒力
基因型菌株对剑尾鱼的致病性研究[J].广东农业科学,2015,42(17):113-117 |
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嗜水气单胞菌毒力因子检测及不同毒力
基因型菌株对剑尾鱼的致病性研究 |
Detection of virulence genes in Aeromonas hydrophila and thepathogenicity of different genotype isolates to swordtail |
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DOI: |
中文关键词: 嗜水气单胞菌 气溶素 溶血素 胞外蛋白酶 细胞毒性肠毒素 毒力基因 剑尾鱼 |
英文关键词: : Aeromonas hydrophila aerolysin(aer) hemolysin A(hlyA) extracellar protease(epa) cytotoxic enterotoxin(act) virulence genes swordtail |
基金项目:国家公益性行业(农业)科研专项(2008
03013) ; 国家科技支撑计划项目(2012BAD25B02) ; 国
家现代农业产业技术体系建设专项(CARS-46) |
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中文摘要: |
选取嗜水气单胞菌(Aeromonas hydrophila, Ah)的 4 种主要胞外毒力因子(气溶素、 溶血素、 胞
外蛋白酶和细胞毒性肠毒)设计合成 4 对引物, 对 2008—2009 年广东、 江西两省的临床分离菌株进行检测,
评价菌株毒力基因的分布特征及其毒力强弱。 60 株临床分离株中, 具有 4 种毒力基因(aer + hlyA + epa + act + )的
菌株占 58.33%(35/60) , 是主要毒力基因型; 其他 25 株的毒力基因有不同数量缺失。 不同基因型的代表菌株
对剑尾鱼攻击试验结果表明, 嗜水气单胞菌毒力因子之间表现出协同作用, aer + hlyA + epa + act + 型的毒力最强, 对
剑尾鱼的致死率最高。 |
英文摘要: |
Four main extracellular virulence factors of Aeromonas hydrophila, including aerolysin(aer) ,
hemolysin(hlyA) , extracellular protease(epa)and cytotoxic enterotoxin(act) , were investigated in 60 isolates
of A. hydrophila by PCR. These 60 stains were isolated from diseased fishes from Jiangxi and Guangdong province
in 2008-2009. The majority(58.33%)of the strains carried the four virulence genes. aer + hlyA + epa + act + virulence
genotype isolates were major in A. hydrophila isolates . 12 representative strains from these genotypes were randomly
selected, and individually injected into swordtail. The results showed that the isolates with more virulence factors had
stronger pathogenicity to swordtail. aer + hlyA + epa + act + genotype isolates were more virulent to the swordtail compared to
others. |
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