叶日英, 王雅玲, 孙力军, 吉宏武, 徐德峰, 代 喆.T-2 毒素对凡纳滨对虾肌肉氨基酸含量
的影响及其灰色关联分析[J].广东农业科学,2015,42(17):118-123 |
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T-2 毒素对凡纳滨对虾肌肉氨基酸含量
的影响及其灰色关联分析 |
Influences of T-2 toxin on amino acids contents of muscle inPenaeus vanmamei and their grey correlation analysis |
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DOI: |
中文关键词: T-2 毒素 凡纳滨对虾 氨基酸 灰色关联分析 |
英文关键词: T-2 toxin Litopenaeus vannamei amino acids grey correlation analysis |
基金项目:国家自然科学基金(31171634, 31371777,
31371746, 31201309) ; 广东海洋大学创新强校工程科研
项目(GDOU2013050205, GDOU2014050203) |
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中文摘要: |
为了探讨对虾肌肉氨基酸含量与饲料中 T-2 毒素暴露剂量的相关性, 利用不同剂量 T-2 毒素的
染毒饲料喂养凡纳滨对虾, 养殖 20 d 后检测各试验组对虾肌肉的 17 种氨基酸含量, 并作氨基酸评分和灰色关
联分析, 从而获得对虾肌肉氨基酸含量的差异程度和相关程度。 氨基酸评分结果显示, 当 T-2 毒素暴露剂量≤
1.2 mg/kg 饲料时, 对虾肌肉必需氨基酸指数升高, 当 T-2 毒素剂量>1.2 mg/kg 饲料时, 必需氨基酸指数则降
低; 灰色关联结果显示, 17 种氨基酸含量的变化与 T-2 毒素剂量均具有显著相关性, 而且每种氨基酸的关联
度非常接近, 其中蛋氨酸与 T-2 毒素剂量具有最大关联。 结果表明, T-2 毒素对对虾肌肉氨基酸具有低剂量刺
激和高剂量抑制的特点, 其中蛋氨酸受抑制影响最大。 |
英文摘要: |
In order to study the amino acids contents of muscle in Penaeus vanmamei exposed to T-2 toxin,
feeds contaminated by different dosages of T-2 toxin were fed to different groups of P. vanmamei, and after 20 days,
the contents of 17 kinds of amimo acids of P. vanmamei in different groups were detected, and then amino acid score
was calculated and grey relational analysis was conducted. The results of amino acid score showed that, when T-2 toxin
content was 1.2 mg/kg feed or less, the essential amino-acid total score increased, and when T-2 toxin content was
above 1.2 mg/kg feed, it reduced. The results of grey correlation analysis showed that there were significant correlations
between 17 kinds of amino acids and T-2 toxin content, and the correlations of 17 kinds of amino acids were very close,
Methionine had the largest correlation with T-2 toxin content. The results indicated that low T-2 toxin content had
stimulation on amino acids of prawn muscle, while high T-2 toxin content had inhibition, and the inhibition effect on
Methionine was the most. |
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