| 侯晓强1，2，任秀艳1，2，付亚娟1，2，乔 洁1，2，张志丽1，吕寒聪1.北沙参内生真菌多样性研究[J].广东农业科学,2015,42(18):20-24 |
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北沙参内生真菌多样性研究 |
| Diversity of endophytic fungi in Glehnia littoralis |
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| DOI： |
| 中文关键词: 北沙参 内生真菌 分离 鉴定 多样性 |
| 英文关键词: Glehnia littoralis endophytic fungi isolation identification diversity |
| 基金项目:河北省教育厅科研项目（2 0 1 1 1 0 8，
Q N 2 0 1 4 1 2 9）；廊坊师范学院微生物学重点学科项目
（2015001） |
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| 摘要点击次数: 2010 |
| 全文下载次数: 582 |
| 中文摘要: |
| ：对北沙参内生真菌类群组成及多样性进行分析。结果表明，从北沙参植株中分离到内生真菌
137 株，利用ITS-rDNA 序列分子鉴定法将北沙参生真菌鉴定为21 个分类单元，分布在6 纲8 目11 科21
属。河北安国北沙参内生真菌归于10 个属，内生真菌的多样性指数H=1.08、D=0.90，优势菌群为链格孢属
（Alternaria）真菌，占总菌株数的29.93%；山东烟台北沙参内生真菌归于17 个属，内生真菌的多样性指数
H=1.31、D=0.94，以刺盘孢属（Colletotrichum）和链格孢属（Alternaria）真菌为优势菌群，分别占总菌株数的
19.71% 和13.87%，两地北沙参内生真菌种类的相似性系数Cs=0.57。由此可见，两地北沙参内生真菌组成具
有一定的相似性，山东烟台北沙参内生真菌多样性高于河北安国北沙参。 |
| 英文摘要: |
| The endophytic fungi were isolated from Glehnia littoralis. The molecular identification of ITSrDNA
was applied to analyze fungal taxa，and fungal diversity was explored. The results showed that 137 fungal
strains were isolated from G. littoralis. These fungi were assigned to 21 taxa，containing 6 classes，8 orders，11
families and 21 generas. Endophytic fungal groups in G. littoralis of Anguo were attributed to 12 generas. Shannon-
Wiener index（H）and Simpson’s index（D）were 1.08 and 0.90 respectively. Alternaria（29.93%）was the
major fungal group. Endophytic fungal groups in G. littoralis of Yantai were attributed to 17 generas. The diversity
index were 1.31（H）and 0.94（D）respectively. Colletotrichum（19.71%）and Alternaria（13.87%）belonged
to the core groups. Thus，the diversity of endophytic fungi in Yantai G. littoralis was higher than that of Anguo G.
littoralis. Moreover，there was a medium similarity（Cs=0.57）of endophytic fungal composition in G. littoralis
between Anguo and Yantai. |
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