刘 飞1,吴成顺1,张 帅2,3,郭 斌1,刘又豪3,曹 庸3.牡蛎水提物抗氧化性研究[J].广东农业科学,2015,42(18):110-114 |
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牡蛎水提物抗氧化性研究 |
Antioxidant activities of aqueous extracts from oystersLIU Fei1,WU Cheng-shun1,ZHANG Shuai2,3,GUO Bin1,LIU You-hao3,CAO Yong3 |
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DOI: |
中文关键词: 牡蛎 水提物 抗氧化性 自由基 |
英文关键词: oyster aqueous extracts antioxidant activity free radical |
基金项目:广东省产学研合作特派员工作站项目(2 0 1 3 B 0 9 0 9 0 0 0 0 5);广东省对外科技合作项目
(2013B051000071) |
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中文摘要: |
以湛江牡蛎为原料,对其水提物抗氧化性进行研究。还原能力测定结果表明,牡蛎水提物具
备一定还原能力,但远低于VC,1.08 mg 牡蛎水提物的还原能力仅相当于28.8 μg Vc。通过对DPPH·、·OH
及·O2- 等3 种自由基清除能力的测定,考察了牡蛎水提物的抗氧化性,结果显示:8 mg/mL 牡蛎水提物溶液
的DPPH·清除率为90.63%,牡蛎水提物清除DPPH·的能力强于VE;0.6 mg/mL 牡蛎水提物的·OH 清除率为
77.23%,牡蛎水提物清除·OH 的能力低于硫脲;高浓度的牡蛎水提物可较好地清除·O2
-。另外,测得牡蛎水提物中短肽占总蛋白含量的50.09%,高含量的短肽可能是牡蛎水提物具备抗氧化活性的主要原因。 |
英文摘要: |
Taking Zhanjiang oyster as materials,the antioxidant activities of aqueous extracts from oysters(AEO)
were researched. The results of reducing capacity determination showed that reducing capacity of 1.08 mg AEO was
only equal to that of 28.8 μg VC,hence,reducing capacity of AEO was far lower than that of Vc. Through determination
of scavenging three free radicals of DPPH·,·OH and ·O2
-,antioxidant activities of AEO were investigated. The
results showed that scavenging rate of DPPH· with 8 mg/mL AEO was 90.63%,and the capacity of scavenging DPPH·
with AEO was higher than that of VE;scavenging rate of ·OH with 0.6 mg/mL AEO was 77.23%,and the capacity of
scavenging ·OH with AEO was lower than that of thiourea;·O2
- could be scavenged with high concentration of AEO.
In addition,oligopeptides content accounted for 50.09% of total protein. High content of oligopeptides maybe the main
cause of antioxidant activities of AEO. |
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