曹 丁1,明飞平1,夏枫耿1,张高贤1,黄乐天1,张玲华2.抗菌肽产生菌的推理选育及抑菌特性分析[J].广东农业科学,2015,42(18):122-126 |
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抗菌肽产生菌的推理选育及抑菌特性分析 |
Rational selection and antibacterial properties analysisof antimicrobial peptides producing bacteriaCAO Ding1,MING Fei-ping1,XIA Feng-geng1,ZHANG Gao-xian1,HUANG Le-tian1,ZHANG Ling-hua2 |
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DOI: |
中文关键词: 抗菌肽 推理选育 抑菌谱 抗菌特性 |
英文关键词: antimicrobial peptide rational selection antimicrobial spectrum antibacterial properties |
基金项目::广州市科技计划项目(2014Y2-00116) |
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中文摘要: |
对一株抗菌肽产生菌GL08 进行选育,采用紫外诱变结合次级代谢产物抗菌肽作为筛选因子的方
法,筛选出一株效价比出发菌株提高30% 的突变菌株。将筛选出的高产菌株进行发酵研究,发现其分泌的抗
菌肽不仅效价稳定,而且抗菌谱广,对多种革兰氏阳性和阴性菌均有较好的杀菌活性。将此菌发酵产物制成粉
末制剂,用不同的温度、pH、蛋白酶和有机溶剂处理,发现其热稳定性好,对不同的pH、蛋白酶和有机溶剂均
有一定的耐受性,且在室温可长期储存且效价不变。通过菌种选育和对抗菌肽制剂的特性分析,发现该抗菌肽
制剂可有望开发成新型饲料添加剂。 |
英文摘要: |
One strain named GL08 which could produce antimicrobial peptides was breeded. By the combination
of ultraviolet mutagenesis and using secondary metabolites- antimicrobial peptides as filter factors,a mutated strain was
screened whose titer was higer than the starting strain by 30%. The fermentation study on the high-yield strain found
that its antimicrobial peptides possessed stable titer,wide antimicrobial spectrum for their good bactericidal activity to
a variety of gram-positive and negative bacteria. The fermented product from the strain GL08 was made into powder that
was treated with different temperatures,pH,proteases and organic solvent,it was found that the antimicrobial peptides
had certain tolerance for different threatments,and a long-term storage at room temperature. By strain breeding and
analysis of the characteristics of antibacterial peptides,the antibacterial peptides from GL08 was expected to develop
into a new type of feed additive. |
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