文章摘要
曹 丁1,明飞平1,夏枫耿1,张高贤1,黄乐天1,张玲华2.抗菌肽产生菌的推理选育及抑菌特性分析[J].广东农业科学,2015,42(18):122-126
查看全文    HTML 抗菌肽产生菌的推理选育及抑菌特性分析
Rational selection and antibacterial properties analysisof antimicrobial peptides producing bacteriaCAO Ding1,MING Fei-ping1,XIA Feng-geng1,ZHANG Gao-xian1,HUANG Le-tian1,ZHANG Ling-hua2
  
DOI:
中文关键词: 抗菌肽  推理选育  抑菌谱  抗菌特性
英文关键词: antimicrobial peptide  rational selection  antimicrobial spectrum  antibacterial properties
基金项目::广州市科技计划项目(2014Y2-00116)
作者单位
曹 丁1,明飞平1,夏枫耿1,张高贤1,黄乐天1,张玲华2 1. 广州市微生物研究所广东 广州 5106632. 华南农业大学生命科学学院广东 广州 510642 
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中文摘要:
      对一株抗菌肽产生菌GL08 进行选育,采用紫外诱变结合次级代谢产物抗菌肽作为筛选因子的方 法,筛选出一株效价比出发菌株提高30% 的突变菌株。将筛选出的高产菌株进行发酵研究,发现其分泌的抗 菌肽不仅效价稳定,而且抗菌谱广,对多种革兰氏阳性和阴性菌均有较好的杀菌活性。将此菌发酵产物制成粉 末制剂,用不同的温度、pH、蛋白酶和有机溶剂处理,发现其热稳定性好,对不同的pH、蛋白酶和有机溶剂均 有一定的耐受性,且在室温可长期储存且效价不变。通过菌种选育和对抗菌肽制剂的特性分析,发现该抗菌肽 制剂可有望开发成新型饲料添加剂。
英文摘要:
      One strain named GL08 which could produce antimicrobial peptides was breeded. By the combination of ultraviolet mutagenesis and using secondary metabolites- antimicrobial peptides as filter factors,a mutated strain was screened whose titer was higer than the starting strain by 30%. The fermentation study on the high-yield strain found that its antimicrobial peptides possessed stable titer,wide antimicrobial spectrum for their good bactericidal activity to a variety of gram-positive and negative bacteria. The fermented product from the strain GL08 was made into powder that was treated with different temperatures,pH,proteases and organic solvent,it was found that the antimicrobial peptides had certain tolerance for different threatments,and a long-term storage at room temperature. By strain breeding and analysis of the characteristics of antibacterial peptides,the antibacterial peptides from GL08 was expected to develop into a new type of feed additive.
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