| 杨明禄1，2，3，陈世有1，.库尔勒香梨的EST-SSR 标记开发
及遗传多样性分析[J].广东农业科学,2015,42(18):127-131 |
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库尔勒香梨的EST-SSR 标记开发
及遗传多样性分析 |
| Development of EST-SSR marker and geneticdiversity analysis of Korla Fragrant Pear |
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| DOI： |
| 中文关键词: 库尔勒香梨 EST-SSR 遗传多样性 |
| 英文关键词: Korla Fragrant Pear EST-SSR genetic diversity |
| 基金项目:新疆生产建设兵团塔里木盆地生物资源保
护利用重点实验室开放课题（BRYB1205）；国家自然科
学基金（31360440） |
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| 摘要点击次数: 1750 |
| 全文下载次数: 686 |
| 中文摘要: |
| 利用已公开的梨EST 序列（2 398 条）开发SSR 引物，分析梨EST 序列中SSR 分布及特征，针对
库尔勒香梨进行引物筛选，并分析受香梨优斑螟为害程度差异较大香梨18 株样本的遗传多样性。结果表明：
EST 序列中有1 813 条无冗余序列，141 条含162 个SSR，占全部EST 的7.78%，出现频率8.94%，平均分布距
离为4.93 kb，但不同微卫星的重复类型间差异很大（9.51~79.87 kb），二核苷酸重复最为常见，占SSR 比例达
到51.85%。从设计的123 对引物中，选取41 对SSR 引物进行筛选，发现17 对引物扩增效果和多态性较好，
共检测出111 条多态性条带，等位基因Na 为5.53，有效等位基因数Ne 为3.74，Nei’s 基因多样性指数He 为
0.69，Shannon’s 多样性指数I 为1.40，受害较重的G 组香梨个体主要集中在UPGMA 聚类的0.78 分支处。 |
| 英文摘要: |
| The SSR primers were developed by reference to the public 2 398 strips of EST sequence in pear，
and by analyzing their distribution and characteristics of SSR markers，then the suitable primers specific to Kuerle
Pear were sorted out，to clarify the genetic diversity of 18 pear trees which showed great variation in damage
level by disoperation of Euzophera pyriella. The results showed that there were 1 813 strips of the non-redundant
sequence in total of 2 398 EST sequence，of which 141 strips contained 162 SSR，amounting to 7.78% of the whole
EST sequence. Their appearance rate was 8.94%，and the average distribution distance was 4.93 kb which showed
extensively difference among microsatellite sequences（from 9.51 to 79.87 kb）. The most common sequence was
the dinucleotide repeat，which accounted for 51.85% of the whole. Choosing 41 pairs from the devised 123 pairs
of primers to conduct primer screening，it was found that there were 111 strips of sequence with polymorphism
bands，among which 17 strips showed favorable amplifying results and polymorphism. The allele Na was 5.53，
and the number of valid allele gene Ne was 3.74，the H value of Nei’sgene diversity（H）was 0.69，and I value of
Shannon’s information index（I）was 40. The individuals suffering serious damage were concentrated on the branch
of 0.78 in the cluster of UPGMA. |
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