文章摘要
赵海霞.氮、磷、钾对不同类型春小麦麦谷蛋白 和HMW-GS 动态形成的影响[J].广东农业科学,2015,42(19):8-13
查看全文    HTML 氮、磷、钾对不同类型春小麦麦谷蛋白 和HMW-GS 动态形成的影响
Effects of nitrogen,phosphorus,potassium onformation of HMW-GS in spring wheat
  
DOI:
中文关键词: 春小麦  HMW-GS  肥力营养
英文关键词: spring wheat  HMW-GS  fertilizer
基金项目:银川能源学院校级科研项目(2013-KYZ- 13)
作者单位
赵海霞 银川能源学院宁夏 银川 750105 
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中文摘要:
      以3 个不同小麦品种中国春、宁春4 号和宁春42 号为材料,在N、P2O5、K2O 不同营养水平下进 行栽培(盆栽)试验,利用SDS-PAGE 电泳等分析技术,研究肥力营养对麦谷蛋白和HMW-GS 亚基动态形成 的影响。结果表明:( 1)在不同水平施肥处理下各品种开花后谷蛋白形成时间不尽相同,但开花后麦谷蛋白 积累变化均呈“低—高—低”总趋势,供试3 个品种随籽粒发育成熟谷蛋白含量逐渐增多,大约在开花后25 d 总含量达最大积累,之后稍有下降;(2)在不同施肥处理水平下3 个品种高分子量谷蛋白亚基花后5 d 开始 至15 d 全部形成,且品种不同亚基形成时间不同,同一品种内亚基形成顺序不同,以Glu-D1x 和Glu-B1x 位 点编码的亚基最早出现,Glu-A1 编码的亚基形成最晚;(3)氮、磷、钾肥影响供试品种宁春4 号、宁春42 号 高分子量谷蛋白亚基的组成。
英文摘要:
      In order to provide theoretical basis for high quality cultivation of wheat,with 3 different wheat varieties,including China spring,Ningchun NO.4 and Ningchun NO.42 as materials,under different timing and stages of nitrogen,phosphorus,potassium application,pot experiments were carried out to study the composition and expression of High Molecular Weight Glutenin Subunit(HMW-GS). The results showed that, in different fertilization treatments,Glutenin formed after flowering time differently,the general trend of Glutenin accumulation changes was dynamic“ low-high-low”. With grain growth and maturity,Glutelin contents of 3 varieties gradually increased,reached the maximum accumulation at about 25 days after flowering,then a slight decline. In different fertilizer treatments,HMW-GSs of 3 varieties all formed in 5-15 days after flowering. The subunit formation time was different in different varieties,and the forming order of HMW-GS was different in the same cultivar,the subunits coded by Glu-D1x and Glu-B1x formed first,while the subunits coded by Glu-A1 formed last. Nitrogen,phosphorus,potassium application had effects on composition of HMW-GS in Ningchun NO.4 and Ningchun NO.42.
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