| 邵 玲，卢夏玲，陈芝敏.濒危植物紫背天葵种质离体保存技术研究[J].广东农业科学,2015,42(19):43-47 |
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濒危植物紫背天葵种质离体保存技术研究 |
| Research on germplasm in vitro preservation technologyof endangered plant Begonia fimbristipula |
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| DOI： |
| 中文关键词: 紫背天葵 离体保存 生长调节剂 基本培养基 濒危植物 |
| 英文关键词: Begonia fimbristipula preservation in vitro growth regulator basic medium endangered plant |
| 基金项目:国家自然科学青年基金（31201142）；广
东省科技计划项目（2010B060200012）；肇庆市科技计划
项目（2013N007） |
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| 摘要点击次数: 1751 |
| 全文下载次数: 609 |
| 中文摘要: |
| 以濒危植物紫背天葵试管苗为材料，研究其种质离体保存的最佳途径。结果表明：常规培养基
6-BA 与NAA 组合并不利于紫背天葵离体种质的保存，保存90 d 后存活率仅有5%。PP333 抑制紫背天葵试管
苗芽丛的分化，显著促进不定根的生成，保存180 d 后，仅3.0 mg/L PP333 存活率达到55%，种质在恢复生长时
有变异现象。低浓度ABA（0.5~2.0 mg/L）具有抑制紫背天葵试管苗生长的作用，保存180 d 后，试管苗存活率
均为100%，且恢复培养时组培苗长势较好。1/2 MS 培养基对紫背天葵试管苗保存效果最好，保存240 d 后存
活率仍高达85%，经恢复生长后，试管苗的增殖及其长势均优于其他处理，可确定为紫背天葵种质离体保存的
最佳方法。 |
| 英文摘要: |
| Taking tube seedlings of endangered plant Begonia fimbristipula as materials，the best way of in vitro
preservation of germplasm was studied. The results showed that conventional medium 6-BA and NAA combination
was not suitable for germplasm in vitro preservation of B. fimbristipula，the survival rate was only 5% after 90 d. PP333
inhibited the differentiation of tube seedlings sprout plexus，significantly promoted the formation of adventitious roots，
after 180 d，only the survival rate of 3.0 mg/L PP333 treatment reached 55%，variation of germplasm in restoring growth.
ABA（0.5-2.0 mg/L）inhibited the growth of tube seedlings，after 180 d，the test-tube seedling survival rate was
100%，the somaclone was better after recovery training. 1/2 MS culture medium had the best effect on B. fimbristipula，
the survival rate was as high as 85% after 240 d. After restoring growth，the tube seedlings multiplication and their
growth were superior to other processings. 1/2 MS culture medium can be used as the best training method for germplasm
in vitro preservation of B. fimbristipula. |
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