| 任 翔，何 德，李翠新，丁浩书.香菇细胞内ITS 致同进化不完全及对
分类鉴定和系统发育的影响[J].广东农业科学,2015,42(19):111-121 |
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香菇细胞内ITS 致同进化不完全及对
分类鉴定和系统发育的影响 |
| Incomplete intracellular ITS concerted evolution in Lentinulaedodes and its influence on identification and phylogenesis |
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| DOI： |
| 中文关键词: nrDNA ITS 香菇 致同进化 系统发育 |
| 英文关键词: nrDNA ITS Lentinula edodes concerted evolution phylogenesis |
| 基金项目:云南省优势特色重点学科生物学建设项目
（50097505）；云南省应用基础研究计划项目（2011FZ141）；
云南省高校特色专业建设点项目（50116001） |
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| 中文摘要: |
| 核糖体DNA 的ITS 序列作为现代分子生物学系统发育及物种鉴定中的一种有效的分子标记被广
泛使用。通过对3 株野生香菇个体细胞总DNA 的提取，nrDNA ITS 序列的扩增、回收、克隆、测序，分析其ITS
序列对构建系统发育树及rRNA 二级结构的影响。结果表明：香菇个体细胞内ITS 序列存在较高的多样性，即
致同进化不完全现象。由此推论ITS 序列并不适用于香菇菌株的鉴定和系统发育研究，而在其他真菌中是否也
有此现象有待进一步研究。 |
| 英文摘要: |
| The internal transcribed spacer（ITS）region of nuclear ribosomal DNA（nrDNA）being one of the
effective molecular markers was widely used for the phylogenesis of modern molecular biology and species identification.
The total DNAs were extracted from the individual cells of three wild Lentinula edodes strains and the nrDNA ITS
sequences were amplified，recovered，cloned and sequenced. Then the influence on the phylogenetic tree construction
and secondary structure of rRNA were analyzed with these ITS sequences. The results showed that there was relatively
high nrDNA ITS diversity in the individual cells of L. edodes，which was suggestive of incomplete concerted evolution.
Therefore this result suggested that the ITS sequence was unsuitable for the research on the phylogenies and species
identification of L. edodes. However，the further research should be carried on whether there exists such the phenomenon
in other fungi. |
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