周国华1,2.水稻冷诱导蛋白激酶基因CIPK07g
的克隆与表达分析[J].广东农业科学,2015,42(21):150-155 |
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水稻冷诱导蛋白激酶基因CIPK07g
的克隆与表达分析 |
Cloning and expression analysis of cold induced proteinkinase gene CIPK07g in rice(Oryza sativa L.) |
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DOI: |
中文关键词: 水稻 冷刺激 蛋白激酶基因 克隆 表达 |
英文关键词: rice cold treatment protein kinase gene clone expression |
基金项目:国家自然科学基金(31200219);云南省
教育厅基金(2012Y371 |
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中文摘要: |
CIPK 是一类Ca2+ 依赖的蛋白激酶超基因家族。对水稻中该基因家族成员之一CIPK07g 进行克
隆,并对其编码蛋白的信号肽、跨膜结构、结构功能域、时空表达特性和低温处理的表达特征进行分析。结果
表明,CIPK07g 基因cDNA 全长1 891 bp,包含279 bp 5′UTR 及259 bp 3′UTR,编码450 个氨基酸组成的多肽
链。该基因蛋白N 端无信号肽,含跨膜结构域,为亲水蛋白,具有CIPK 基因家族特有的保守结构域,在叶片
中表达较高,低温刺激后24 h 表达达到高峰。 |
英文摘要: |
The gene CIPK07g in rice was cloned,which was one of the members of Ca2+-dependent protein kinase
superfamily,and it’s signal peptide,transmemberane domain,conserved domain,expression profile in different
organisms and low temperature treatment were analyzed. The results showed that,the cDNA full length of CIPK07g gene
was 1 891 bp,including 279 bp 5′UTR and 259 bp 3′UTR,which encoded a polypeptide with 450 amino acids. There
was a characteristic conserved domain of CIPK gene family in CIPK07g protein,and the protein was a hydrophilicity
protein which included a transmemberane domain but had no signal peptide in N-terminal analyzed by bioinformatic
tools. The CIPK07g gene was highly expressed in leaf after 24 h cold treatment. |
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