| 吴秋生1，2，宋 菲2，黄玉林2，王 挥2，赵松林2，陈卫军2.大孔树脂分离纯化槟榔花多酚研究[J].广东农业科学,2015,42(23):122-126 |
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大孔树脂分离纯化槟榔花多酚研究 |
| Separation and purification of polyphenols fromareca inflorescence by macroporous resin |
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| DOI： |
| 中文关键词: 槟榔花 多酚 大孔树脂 分离纯化 |
| 英文关键词: areca inflorescence polyphenols macroporous resin separation and punification |
| 基金项目:海南省自然科学基金（314146） |
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| 中文摘要: |
| 采用大孔吸附树脂对槟榔花多酚进行分离纯化，确定其分离纯化条件。通过静态吸附试验和动态
吸附解吸试验，考察AB-8、SP700、SP850、XAD-7HP、D101 和HP2MG 等6 种型号树脂对槟榔花多酚的吸附
量和解吸率，筛选出吸附效果最好的树脂，并得出最佳的吸附条件。结果表明，AB-8 树脂的吸附和解吸效果
最好，可以用准二级动力学方程较好地描述AB-8 树脂对槟榔花多酚的吸附。最佳的分离纯化条件为：槟榔花
多酚粗提液pH 为4，上样初始浓度为0.4 mg/mL，洗脱剂乙醇浓度为60%，上样流速和洗脱流速均为3 BV/h。
AB-8 型大孔树脂在所确定的工艺条件下，树脂的吸附—解吸附性能稳定，且能较好地分离纯化槟榔花多酚，多
酚纯度在纯化前为2.7%、纯化后为34.6%。 |
| 英文摘要: |
| Macroporous resins were used to separate and purify polyphenols from the extract of areca
inflorescence，and the conditions of separation and purification were determined. The adsorption capacity and desorption
rate of six kinds of resins，SP850，XAD-7HP，SP700，D101 and HP2MG，were compared to select the most suitable
one，through static adsorption experiment and dynamic adsorption and desorption experiment. The results showed
that AB-8 resin was the best one and the kinetic data fitted the pseudo-second-order kinetics model better. Optimal
conditions were as follows：the sample pH was 4.0，concentration of ethanol used as elution agent was 60%，initial
sample concentration was 0.4 mg/mL，adsorption rate and desorption rate was 3 BV/h. AB-8 resin performed great in
separating and purifying polyphenols from areca inflorescence，and the purification of polyphenols was 2.7% at first and
increased to 34.6% after purification. |
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