| 谭 华，邹成林，吴永升，郑德波，莫润秀，黄爱花，韦新兴，蒋维萍，韦 慧，黄开健.不同遗传背景普通玉米种质导入
opaque-2 基因效应探讨[J].广东农业科学,2015,42(23):127-132 |
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不同遗传背景普通玉米种质导入
opaque-2 基因效应探讨 |
| Effect of introducing opaque-2（o2）gene to normal maizegermplasm with different genetic background |
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| DOI： |
| 中文关键词: ：普通玉米 o2 基因 遗传效应 |
| 英文关键词: normal maize o2 gene genetic effect |
| 基金项目:广西自然科学基金（2 0 1 3 G X N S F A A
019101）；广西重大计划专项（桂科重14121001-1-9）；
广西科技攻关项目（桂科攻10100005-3）；广西农业科学
院青年科技专项（桂农科2013JQ02） |
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| 中文摘要: |
| 利用杂交转导把优质蛋白玉米控制赖氨酸合成的Opaque-2 基因（o2 基因）导入普通玉米种质，
借助分子标记辅助选择创制o2 近等基因系，探讨o2 基因的遗传效应。结果表明，不同遗传背景普通玉米种质
导入o2 基因能大幅度提高第一限制性氨基酸赖氨酸含量，其他氨基酸含量也发生变化；热带、亚热带背景种
质百粒重有所下降、百粒体积略有提高；温带背景种质百粒重及百粒体积都有较大幅度提高。研究认为，利用
不同遗传背景普通玉米种质导入o2 基因是扩增优质蛋白玉米基因源的有效方法。 |
| 英文摘要: |
| Introducing opaque-2 gene into normal maize germplasm by molecular assisted selection（MAS）was
a popular technique in maize breeding nowadays. In this study，several near-isogenic lines developed by MAS were
used to discuss the genetic effect of o2 gene. The results showed that introducing o2 gene into normal maize germplasm
with different genetic background could improve lysine（which is called the first restrictive amino acid）and other
amino acids contents significantly. 100-kernel weight decreased and 100-kernel volume increased slightly when o2
gene was introduced into tropical and subtropical maize germplasm. However，100-kernel weight and 100-kernel
volume increased greatly when o2 gene was introduced into temperate germplasm. Introducing o2 gene into normal
maize germplasm with different genetic background was a good way for germplasm enhancement in quality protein
maize（QPM）. |
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