| 曹红星1，雷新涛1，刘艳菊1，孙程旭1，张如莲2.椰子抗寒相关生理生化指标筛选及评价[J].广东农业科学,2016,43(2):49-54 |
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椰子抗寒相关生理生化指标筛选及评价 |
| Identification and comprehensive evaluation of cold resistance indexes of coconut |
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| DOI： |
| 中文关键词: 关键词：低温胁迫 椰子 生理生化指标 |
| 英文关键词: Key words：low temperature stress coconut physiological and biochemical index |
| 基金项目: |
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| 摘要点击次数: 2526 |
| 全文下载次数: 1058 |
| 中文摘要: |
| 以2 年生海南高种椰子、红矮椰子和香水椰子3 个椰子品种的幼苗为材料，根据温度的日变化规律，设置不同的低温度处理，分别为CK（自然温度处理）、16℃（T1 处理）、12℃（T2 处理）、8℃（T3 处理），利用相关性分析、聚类分析和主成分分析法研究了低温胁迫下椰子叶片MDA 含量、可溶性糖含量、可溶性蛋白质含量、游离脯氨酸含量、超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性和抗坏血酸过氧化物酶（APX）活性的变化，对抗寒密切相关的生理生化指标进行筛选，对不同椰子品种的抗寒性进行评价。结果表明：根据不同品种抗寒性相关生理生化指标变化幅度的不同，抗寒性强弱依次为海南高种椰子＞红矮椰子＞香水椰子。脯氨酸含量和POD 活性与其他指标相关性高，不同低温处理间差异较大，可以聚为不同类分别代表渗透调节物质和保护酶类的指标，主成分分析结果表明，这2 个指标可反映其他指标信息，可作为椰子抗寒鉴定指标。 |
| 英文摘要: |
| Taking two years old Hainan tall coconut，red dwarf coconut and aromatic coconut seedlings as materials，four low temperature treatments，including CK（ natural temperature treatment），16℃（ T1 treatment），12℃（ T2 treatment），8℃（ T3 treatment） were designed according to the daily temperature change regularity. After treated for 21 days，the changes of malondialdehyde （MDA）content，soluble sugar content，soluble protein content，free proline content，superoxide dismutase（ SOD） activity，peroxidase（ POD） activity，catalase（ CAT） activity，ascorbate peroxidase（ APX） activity were analyzed. The data were treated by pertinence analysis，clustering analysis，principal component analysis for cold resistance indexes identification and their comprehensive evaluation of cold tolerance. The results showed that there were different change tendency of physiological and biochemical traits under low temperature treatments in different coconut varieties，the cold resistance of the three varieties were listed as Hainan tall coconut ＞ red dwarf coconut ＞ aromatic coconut. Free proline content and POD activity were highly related with other indexes，there were greater differences under different low temperature treatments，they were clustered into different
classes and indicated as osmotic substance and protective enzyme index，respectively. These two indexes could reflect the information of other indexes according to the principal component analysis. Free proline content and POD activity could be identified as cold resistance evaluation indexes of coconut. |
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