马兰根黄酮类化合物的提取工艺及其对毛尖茶叶脂肪氧合酶的抑制作用

吴桂玲1; 罗德尉2

文章摘要

吴桂玲1，罗德尉2.马兰根黄酮类化合物的提取工艺及其对毛尖茶叶脂肪氧合酶的抑制作用[J].广东农业科学,2016,43(2):123-127

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Extraction technology of flavonoids in Kalimeris indica root and its inhibitory Effects on activity of Lipoxygenase of Maojian tea leaves

DOI：

中文关键词: 采用正交试验，对马兰根中总黄酮提取条件进行优选，并研究了提取的马兰根黄酮化合物对毛尖茶叶脂肪氧合酶LOX 的抑制作用。结果表明，乙醇体积分数70％、提取时间为2.5 h、固液比为1∶20 g/mL，提取效率较好，最佳工艺条件下黄酮提取率为16.422％马兰根中黄酮化合物和BHA、BHT 及Vc 均对LOX 有抑制作用，IC50 分别为34.282、10.938、9.049、9.04 μg/mL。

英文关键词: Kalimeris indica root flavonoids orthogonal design Maojian tea leaves lipoxygenase inhibitory effects

基金项目:

作者	单位
吴桂玲1，罗德尉2	（1. 贵州省黔南民族师范学院化学化工学院，贵州都匀 558000；2. 贵州高速黔通建设工程有限公司，贵州都匀 558000）

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中文摘要:

采用正交试验，对马兰根中总黄酮提取条件进行优选，并研究了提取的马兰根黄酮化合物对毛尖茶叶脂肪氧合酶LOX 的抑制作用。结果表明，乙醇体积分数70％、提取时间为2.5 h、固液比为1∶20 g/mL，提取效率较好，最佳工艺条件下黄酮提取率为16.422％；马兰根中黄酮化合物和BHA、BHT 及Vc 均对LOX 有抑制作用，IC50 分别为34.282、10.938、9.049、9.04 μg/mL。

英文摘要:

The optimal extraction conditions of flavonoids in Kalimeris indica root were determined by orthogonal design. The results showed that the optimal conditions were as follows: 70% ( volume fraction) ethanol，extraction time of 2.5 h， 1∶20（g/mL） solid-liquid ratio，the yield of total flavonoids could reach 16.422%. The inhibitory effects of flavonoids from root of K. indica on the activity of lipoxygenase of Maojian tea leaves were also studied，and were compared with the same concentration of BHA， BHT and Vc. The results showed that flavonoids from root of K. indica，BHA， BHT and Vc all had inhibitory effects on the activity of lipoxygenase，IC50 were 34.282 μg/mL， 10.938 mg/mL，9.049 μg/mL and 9.04 μg/mL.

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