| 杨 颖1，施巧婷2，陆 江3，石德顺1，徐照学2，李湘萍.西门塔尔牛HDAC1 基因SNP 检测及其与屠宰性状的相关性分析[J].广东农业科学,2016,43(2):128-132 |
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西门塔尔牛HDAC1 基因SNP 检测及其与屠宰性状的相关性分析 |
| Detection of gene HDAC1 SNP and its relationship with slaughtering traits in Simmental cattle |
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| DOI： |
| 中文关键词: 西门塔尔牛 组蛋白去乙酰化酶1 单核苷酸多态性 屠宰性状 |
| 英文关键词: Simmental cattle histone deacetylase1 single nucleotide polymorphism slaughtering traits |
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| 中文摘要: |
| 通过对37 头西门塔尔牛第2 条染色体上的组蛋白去乙酰化酶1 基因（HDAC1）的Intron 4-Intron5、Intron 10-Intron 11 和3′非翻译区进行克隆和序列分析，采用SSCP 分析方法和PCR 产物直接测序的方法，判定SNP 基因型，并分析SNP 基因型与屠宰性状的相关性。结果发现，西门塔尔牛的HDAC1 基因Intron4-Intron 5 以及3′端非翻译区没有突变位点；在HDAC1 基因Intron 10-Intron 11 区存在1 个突变位点，该突变位点位于测序结果的110 bp 处，为A/G 突变，其中AG 杂合子频率（48.6%）与纯合子GG/AA 频率（51.4%）大致相当，但G 等位基因频率（62%）远大于A 等位基因出现的频率（38%）。经在37 头西门塔尔牛屠宰样品中的背膘厚屠宰性状比较，AA 基因型个体的背膘厚显著低于GG 和AG 型个体，提示G 等位基因对生长具
有正效应。 |
| 英文摘要: |
| In this study，we cloned，sequenced and analyzed the regions of Intron 4-Intron 5，Intron 10-Intron 11 and 3'-non of gene Histone deacetylase 1（ HDAC1） on the second chromosomes in 37 Simmental cattles. By the methods of PCR-SSCP and sequencing analysis，we tried to judge and determine their SNP genotypes，and explore the relationship between SNP genotypes and slaughter traits in Simmental cattle. The results indicated that，there was no mutation on the regions of Intron 4-Intron 5 and 3'-non-translation of gene HDAC1 in Simmental cattle，but there was one mutation in the region of Intron 10-Intron 11 of gene HDAC1，and it located on the 110 bp polymorphism site of PCR sequencing result，it was a A/G mutation，the frequency of allele G（ 62%） was more than allele A（38%）. By comparison and analysis，it might be co-related between different allele A/G genotype on region of Intron 10-Intron 11 and backfat thickness（ measured） of slaughtering traits of Simmental cattle. There was significantly lower backfat thickness（ measured） of slaughtering traits in genotype of AA than that in genotype of GA or GG. According to the results，in this 110 bp location of region Intron 10-Intron 11 of gene HDAC1，allele G may perform a positive effect on the backfat thickness（measured） of Slaughtering traits in simmental cattle. |
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