| 王金宾，金东昊，黄成成，孙浩然，陈 帅，李绪彦.大豆GmMYB138A 基因的克隆
及冷胁迫诱导表达分析[J].广东农业科学,2016,43(6):43-48 |
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大豆GmMYB138A 基因的克隆
及冷胁迫诱导表达分析 |
| Cloning of GmMYB138A from Glycine max. andits expression analysis under cold stress |
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| DOI：10.16768/j.issn.1004-874X.2016.06.009 |
| 中文关键词: 大豆 GmMYB138A 克隆 冷胁迫 基因表达 |
| 英文关键词: soybean GmMYB138A cloning cold stress gene expression |
| 基金项目:国家自然科学基金（31300253）；国家大
学生创新创业训练计划项目（2014A82371） |
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| 中文摘要: |
| 根据大豆基因组信息，从大豆品种吉林32 中克隆了1 个转录因子基因，该基因对应大豆基因组
中的CDS Glyma02g03020。生物信息学分析表明，该基因编码的蛋白属于MYB 转录因子类型，主要分布在细
胞核中；进化树分析将它与1R-MYB 蛋白聚为一类，并与大豆MYB138 亲缘关系最近，同源性可达96%，将其
命名为GmMYB138A；进一步利用qRT-PCR 分析GmMYB138A 响应冷胁迫的表达模式，结果表明随着胁迫处
理时间的延长，该基因表达逐渐增加，并在胁迫24 h 达到高峰，提示GmMYB138A 可能参与大豆的冷胁迫应答
过程。 |
| 英文摘要: |
| According to the soybean genomic database，a transcription factor gene，GmMYB138A，was cloned
from Glycine max. cv Jilin32. Bioinformatic analysis indicated that the protein encoded by this gene belonged to 1RMYB
group，and was localized in nucleus. Sequence alignment and phylogenetic analysis showed that GmMYB138A
was clustered into the 1R-MYB group，evolutionarily closest to MYB138 from soybean，and the identity was up to 96%.
Furthermore，q PCR analysis was conducted to examine its expression pattern in response to cold stress. As a result，the
transcriptional level was gradually promoted with the cold treatment duration，and the peak occurred at 24h after cold
stress，suggesting that GmMYB138A might be involved in the signaling transduction during cold stress. |
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