文章摘要
杨 红1,张 勇2,何 琦3,李 俊2,张 雄2,彭 松2.3 个鸡种NKX2-5 基因多态性 与胫长的关联性分析[J].广东农业科学,2016,43(6):156-162
查看全文    HTML 3 个鸡种NKX2-5 基因多态性 与胫长的关联性分析
Polymorphism and association analysis of NKX2-5 genewith shank length in three chickens
  
DOI:10.16768/j.issn.1004-874X.2016.06.027
中文关键词: NKX2-5 基因    胫长  SSCP  多态性
英文关键词: NKX2-5 gene  chicken  shank length  SSCP  polymorphism
基金项目:贵州省农业动植物育种专项( 黔农 育专字[2012]10 号);浙江大学基本科研业务费专项 (2012XZZX003-1)
作者单位
杨 红1,张 勇2,何 琦3,李 俊2,张 雄2,彭 松2 1. 毕节市农业经营管理站贵州 毕节 551700 2. 贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室/ 贵州大学动物科学学院/ 贵州省动物遗传育种与繁殖重点实验室贵州 贵阳 550025 3. 贵阳市花溪区农业局贵州 贵阳 550025 
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中文摘要:
      以贵州特有鸡种高脚鸡、矮脚鸡和百宜黑鸡为试验对象,利用PCR-SSCP 和DNA 测序等方法检 测了NKX2-5 基因T563C、C675T 位点在3 个试验鸡群中不同基因型与胫长的关联性。T563C 与胫长关联分析 表明,高脚鸡群体中TT 基因型个体胫长显著高于CC 型,百宜黑鸡群体中TT 基因型显著高于CT 型,矮脚鸡 群体中不同基因型个体间胫长差异不显著;C675T 突变位点在3 个鸡种中只有CC 和CT 两种基因型,且各鸡 种不同基因型个体间胫长差异不显著。
英文摘要:
      Gaojiao chicken,Aijiao chicken and Baiyi black chicken in Guizhou province were used as experimental subjects in this study. SNPs of NKX2-5 genes were detected in three chicken vareties by PCR-SSCP and DNA sequencing technologies. The 2 SNPs were detected in the 3 chicken groups,T563C,C675T sites of NKX2-5 gene were missense mutations,The relevance between T563C site of NKX2-5 gene and chicken shank length showed that,TT genotype was significantly higher than CC genotype among Gaojiao chicken groups(P < 0.05),TT genotype was significantly higher than CT genetype in Baiyi black chicken groups(P < 0.05),uhile no significant level aruong 3 genotypes in Aijiao chicken. The C675T site of NKX2-5 had only CC and CT genotypes,and there was no significant level in different genotypes of 3 vareties.
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