杨 红1,张 勇2,何 琦3,李 俊2,张 雄2,彭 松2.3 个鸡种NKX2-5 基因多态性
与胫长的关联性分析[J].广东农业科学,2016,43(6):156-162 |
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3 个鸡种NKX2-5 基因多态性
与胫长的关联性分析 |
Polymorphism and association analysis of NKX2-5 genewith shank length in three chickens |
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DOI:10.16768/j.issn.1004-874X.2016.06.027 |
中文关键词: NKX2-5 基因 鸡 胫长 SSCP 多态性 |
英文关键词: NKX2-5 gene chicken shank
length SSCP polymorphism |
基金项目:贵州省农业动植物育种专项( 黔农
育专字[2012]10 号);浙江大学基本科研业务费专项
(2012XZZX003-1) |
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中文摘要: |
以贵州特有鸡种高脚鸡、矮脚鸡和百宜黑鸡为试验对象,利用PCR-SSCP 和DNA 测序等方法检
测了NKX2-5 基因T563C、C675T 位点在3 个试验鸡群中不同基因型与胫长的关联性。T563C 与胫长关联分析
表明,高脚鸡群体中TT 基因型个体胫长显著高于CC 型,百宜黑鸡群体中TT 基因型显著高于CT 型,矮脚鸡
群体中不同基因型个体间胫长差异不显著;C675T 突变位点在3 个鸡种中只有CC 和CT 两种基因型,且各鸡
种不同基因型个体间胫长差异不显著。 |
英文摘要: |
Gaojiao chicken,Aijiao chicken and Baiyi black chicken in Guizhou province were used as
experimental subjects in this study. SNPs of NKX2-5 genes were detected in three chicken vareties by PCR-SSCP and
DNA sequencing technologies. The 2 SNPs were detected in the 3 chicken groups,T563C,C675T sites of NKX2-5
gene were missense mutations,The relevance between T563C site of NKX2-5 gene and chicken shank length showed
that,TT genotype was significantly higher than CC genotype among Gaojiao chicken groups(P < 0.05),TT genotype
was significantly higher than CT genetype in Baiyi black chicken groups(P < 0.05),uhile no significant level aruong
3 genotypes in Aijiao chicken. The C675T site of NKX2-5 had only CC and CT genotypes,and there was no significant
level in different genotypes of 3 vareties. |
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