| 黄勋和，张金枫，谭坤凤，李丽芝，李威娜，钟福生.五华三黄鸡MHC-B 区域复合微卫星位点
LEI0258 遗传多样性与进化研究[J].广东农业科学,2016,43(6):163-168 |
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五华三黄鸡MHC-B 区域复合微卫星位点
LEI0258 遗传多样性与进化研究 |
| Diversity and evolution of the highly tandem repeat LEI0258with in MHC-B region in Wuhua three-yellow chicken |
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| DOI：10.16768/j.issn.1004-874X.2016.06.028 |
| 中文关键词: 多态性 主要组织相容性复合体 LEI0258 五华三黄鸡 进化 血清学 |
| 英文关键词: polymorphism major histocompatibility complex LEI0258 Wuhua three-yellow chicken evolution serology |
| 基金项目:广东省自然科学基金（2 0 1 4 A 0 3 0 3 0
7018）；嘉应学院“创新强校工程”项目（CQX019）；
广东省公益研究与能力建设项目(2015A020208020，
2 0 1 6 A 0 3 0 3 0 3 0 6 8 ) ；嘉应学院重点科技计划项目
（2015KJM03） |
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| 摘要点击次数: 1884 |
| 全文下载次数: 746 |
| 中文摘要: |
| 应用MHC-B 区域复合微卫星位点LEI0258 研究五华三黄鸡的遗传多样性与进化地位。147
份样品共检测到48 个等位基因，长度范围为181~495 bp。等位基因251（0.0952）、275（0.0850）和310
（0.0816）频率最高。DNA 测序鉴定出44 个等位基因，其中42 个为首次发现。观察杂合度、期望杂合度和多
态信息含量分别为0.830、0.927 和0.952。9 个等位基因与15 种已知MHC 血清型相匹配。基于侧翼区变异信
息的中介网络图将44 个等位基因分为7 个进化枝，其中3 个进化枝存在于红原鸡中，提示五华三黄鸡起源于
红原鸡。研究结果表明，微卫星LEI0258 适用于鸡群体遗传多样性、血清型鉴定和进化研究。 |
| 英文摘要: |
| To investigate the polymorphism and evolution of Wuhua three-yellow chicken，the tandem repeat
LEI0258 located within the B region of chicken major histocompatibility complex（ MHC-B region） was applied. A total
of 48 alleles ranging from 181 to 495 bp in 147 samples were found. Three alleles，251（ 0.095），275（ 0.0850），
and 310（ 0.0816） were the most frequency in this breed. Forty-two of 44 alleles defined by DNA sequencing was novel
for Wuhua three-yellow chicken. The LEI0258 showed high levels of polymorphism，with 0.830，0.927 and 0.952 for
observed heterozygosity，expected heterozygosity and polymorphic information content，respectively. Nine alleles were
corresponding to 15 available serotypes. The 44 alleles were classified into seven clusters，based on the SNPs and indels
found within the sequences flanking the repeats，of which three was also found in red fowl jungle，indicating that Wuhua
three-yellow chicken was of origin from red fowl jungle. The results presented herein suggested that LEI0258 could be
used as a candidate molecular marker for uncover genetic diversity，serology and evolution in chicken. |
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