| 谭 华，邹成林，郑德波，韦新兴，黄爱花，蒋维萍，韦 慧，黄开健.分子标记辅助选择抗南方玉米锈病材料[J].广东农业科学,2016,43(7):6-10 |
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分子标记辅助选择抗南方玉米锈病材料 |
| Selection of materials with resistance to southern corn rust（SCR） by marker-assistant selection（MAS） in maize（Zea mays L.） |
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| DOI：10.16768/j.issn.1004-874X.2016.07.002 |
| 中文关键词: 南方玉米锈病 分子标记辅助选择 抗锈改良 |
| 英文关键词: southern corn rust（ SCR） marker-assistant selection（ MAS） SCR-resistant improvement |
| 基金项目:广西重大科技计划专项（桂科重14121001-1-9）；广西自然科学基金（2013GXNSFAA019101） |
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| 中文摘要: |
| 以来自国际玉米小麦改良中心（CIMMYT）的高抗南方玉米锈病种质为抗锈源，把抗病基因导入来自温带欠抗锈种质，借助分子标记辅助选择，聚合抗锈基因定向改良，对获得的BC2F2 进行田间抗病性接种鉴定。结果显示，利用phi048 选择与抗病亲本相同带型的BC2F2 家系368 个单株接种鉴定，抗及高抗级别植株共235 株，占总株数的63.9%；中抗级别植株114 株，占31.0%；感病植株19 株，占5.1%。表明利用phi048 对南方玉米锈病抗病材料进行早代选择是有效的，可淘汰大量感病家系，减轻田间工作量；利用分子标记辅助定向选择结合抗病接种鉴定，能有效改良温带种质的抗锈性。 |
| 英文摘要: |
| In order to improve the maize resistance to southern corn rust of temperate germplasm，the resistant genes，which came from germplasm of high SCR-resistant in CIMMYT，were introduced in temperate germplasm.The SCR-resistance of temperate germplasm was directionaly improved by pyramiding SCR-resistant genes. 368 plants of a BC2F2 family，which had the same polymorphism with the SCR-resistant parent by detecting with SSR marker phi048，were used to identify their SCR-resistance by artificial inoculation in field. The results showed that 235 plants were highly resistant or resistant，accounting for 63.9%，114 plants were moderately resistant，accounting for 31.0%，19 plants were susceptible，accounting for 5.1%. The results demonstrated that SSR marker phi048 was effective to select SCR-resistant materials in early stage，which could knock out lots of SCR-susceptible lines therefore reduce plenty of field work. The SCR-resistance of temperate germplasm was effectively improved by directional selection with marker assistant combined to identification with artificial inoculation. |
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