| 李 宁，刘炳梅，陈雪梅.油菜素内酯合成基因DWF1、DET2 影响毛白杨木质部形成[J].广东农业科学,2016,43(7):51-56 |
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油菜素内酯合成基因DWF1、DET2 影响毛白杨木质部形成 |
| Influences of brassinolide synthesis gene DWF1 and DET2 on xylem formation in Populus tomentosa |
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| DOI：10.16768/j.issn.1004-874X.2016.07.009 |
| 中文关键词: 毛白杨 油菜素内酯合成基因 木质部 细胞壁组成 |
| 英文关键词: Populus tomentosa brassinolides synthesis gene xylem cell wall composition |
| 基金项目: |
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| 摘要点击次数: 2220 |
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| 中文摘要: |
| 油菜素内酯对植物的生长发育有重要的调控作用。以转油菜素内酯合成基因GhDWF1 和
GhDET2 的毛白杨为材料，对其生长、木质部显微结构、细胞壁化学组成进行分析，发现油菜素内酯合成基
因DWF1 和DET2 基因的转入影响了毛白杨的木质部形成和细胞壁组分。转基因毛白杨的木质部面积明显
高于对照，且细胞较小，细胞壁较厚，可以推测毛白杨体内细胞的分裂和横向扩大受到影响；导管上的纹孔
增大，可能会影响水分运输；酸性果胶升高，纤维素含量降低，可能改变了细胞壁的框架结构；转GhDET2
基因的毛白杨的木质素含量较高，S 型木质素含量较高，化学降解相对容易；而转GhDWF1 的毛白杨的木质
素含量较低，G/S 比最高，S 型木质素含量较小。 |
| 英文摘要: |
| Brassinolides are important for plants growth and development. Transgenic poplars with brassinolides
biosynthesis genes GhDWF1 and GhDET2 were selected and analyzed about the growth，microstructure of xylem
and cell wall composition. It was found that DWF1 and DET2 had influences on xylem formation and cell wall
composition in Populus tomentosa. Transgenic poplars had higher xylem area，smaller cells and thicker cell walls
than the control，which may affect cell division and lateral expansion. Pits on vesselswere enlarged，which may affect
water transport in poplars. Contents of acidic pectin increased，and contents of cellulose decreased in transgenic
poplars， which may change frame structures of cell wall. Transgenic poplars with GhDET2 had higher lignin content
and high S-lignin ratio， which may cause chemical degradation easily，while transgenic poplars with GhDWF1 had
lower lignin content，higher G/S ratio and smaller S-lignin content . |
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