文章摘要
徐文娴1,赵从举1,朱敏捷1,吴喆滢2,韩 奇1,郑庆扬1.海南岛桉树人工林土壤微生物功能多样性特征[J].广东农业科学,2016,43(7):65-72
查看全文    HTML 海南岛桉树人工林土壤微生物功能多样性特征
Soil microbial functional diversity of Eucalyptus plantation in Hainan Island
  
DOI:10.16768/j.issn.1004-874X.2016.07.011
中文关键词: Biolog-ECO 微平板  桉树人工林  功能多样性  土壤微生物
英文关键词: Biolog-ECO microplate  Eucalyptus plantation  functional diversity  soil microbe
基金项目:国家自然科学基金(41361006);国家科技支撑计划项目(2012BAC18B04)
作者单位
徐文娴1,赵从举1,朱敏捷1,吴喆滢2,韩 奇1,郑庆扬1 (1. 海南师范大学地理与旅游学院海南 海口 5711582. 海南师范大学生命科学学院海南 海口 571158) 
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中文摘要:
      运用 Biolog-ECO 微平板技术,对海南岛4 龄桉树人工林不同土层深度的土壤微生物群落功能多样性的动态变化进行分析。结果表明:随着培养时间的延长,土壤微生物平均AWCD 逐渐增大,代谢活性逐渐增强,300 h 时AWCD 值下层土> 中层土> 上层土;不同土层微生物对胺类碳源利用率最高,酚酸 类、氨基酸类和多聚物类次之,碳水化合物和羧酸类最低;随着土层深度增加Shannon 多样性指数(H)逐渐增大,20~30 cm McIntosh 丰富度指数(U)和Simpson 优势度指数(D)明显高于其他土层,而McIntosh均匀度指数(E)又显著较低,0~10 cm 土层的Shannon 多样性指数(H)、Simpson 优势度指数(D)和McIntosh 丰富度指数(U)均较低,即桉树人工林表层土壤的微生物活性受到不同程度的抑制;主成分分析(PCA)表明,在31 种碳源中提取的2 个主成分因子分别可以解释所有变量方差的41.65% 和32.51%,对PC1 和PC2 起分异作用的主要碳源为碳水化合物、氨基酸及其衍生物类和胺类;土壤微生物功能多样性还受土壤性状的综合调控,尤其与土壤有机质、土壤容重和土壤碱解氮含量相关性最明显。
英文摘要:
      As one of the most important components in soil ecosystem,soil microbes have irreplaceable effects on nutrient and energy cycling. In the research,the changes of functional diversity of soil microbial community were analyzed by using the method of Biolog-ECO microplate culture in different soil depths of 4-year Eucalyptus plantation in western Hainan Island. The results showed that the average well color development( AWCD) increased with incubation time,and there were significant differences among different soil layers,varying from 0.85,0.97 and 1.00(cultured for 300 hours) for 0-10 cm,10-20 cm and 20-30 cm soil layer. The microbial communities in different soil layers had similar carbon sources utilization mode; it found that amine was the highest availability of carbon sources,followed by Phenolic acids,amino acids,polymer,and carbohydrates,and carboxylic acids had the lowest utilization rate. However,the carbon utilization efficiency increased with the soil depth,the values of Shannon-Wiener index( H),Simpson index( D) and McIntosh index( U) all increased with layer increasing; it
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