| 陈伟国1，李广伟2，严专强2，谢青梅1.J 亚群禽白血病病毒受体基因NHE1 的遗传多态性分析[J].广东农业科学,2016,43(7):145-150 |
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J 亚群禽白血病病毒受体基因NHE1 的遗传多态性分析 |
| Analysis on genetic polymorphism of avian leukosis virus subgroup J receptor gene NHE1 |
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| DOI：10.16768/j.issn.1004-874X.2016.07.022 |
| 中文关键词: J 亚群禽白血病病毒 NHE1 受体基因 序列分析 遗传多态性 |
| 英文关键词: ALV-J NHE1 receptor gene sequence analysis genetic polymorphism |
| 基金项目:广东省自然科学基金团队项目（S2013030013313） |
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| 摘要点击次数: 1893 |
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| 中文摘要: |
| 为发掘J 亚群禽白血病病毒（ALV-J）受体基因NHE1 潜在的遗传抗性位点，采用PCR 技术分段扩增NHE1 受体基因序列，并分析其遗传多态性。结果显示，在黄羽肉鸡NHE1 基因外显子区域发现了16 个SNP 突变，分别为c.309 G>C，c.11653 C>T，c.11689 G>A，c.11917 C>T，c.11946 C>T，c.13405 C>A，c.13429 T>C，c.13438 T>C，c.14108 A>C，c.14516 A>G，c.14522 G>A，c.14654 C>T，c.15834 G>A，c.15873G>A，c.17560 C>G and c.17770 G>A。进一步分析表明这些SNP 突变均为同义cSNP，未引起氨基酸发生改变；在黄羽肉鸡NHE1 基因内含子区域发现了SNP 和插入/ 缺失突变，NHE1 基因内含子2 c.12239～12240和c.13114～13115 分别存在AGGGCTGC 和TGAAGCC 插入突变，内含子11 c.17073～17091 存在TGTCCTGTGTCCTCCCTGC 缺失突变；在ALV-J 攻毒后阴性鸡和阳性鸡的NHE1 基因外显子区域均发现c.309 G>C、c.11653 C>T、c.13405 C>A、c.13438 T>C、c.14108 A>C 和c.14516 A>G 6 个SNP 突变，内含子区域也均发现与黄羽肉鸡相同的插入/ 缺失突变。研究结果表明，发现的NHE1 受体基因遗传变异位点不能作为ALV-J 潜在的遗传抗性位点。 |
| 英文摘要: |
| To explore the potential genetic resistance loci in avian leukemia virus subgroup J（ ALV-J） receptor gene NHE1，the sequence of NHE1 receptor gene was amplified by PCR technique，and the genetic polymorphism of NHE1 gene was analyzed. The results indicated that 16 SNP mutations，including c.309 G>C，c.11653 C>T，c.11689 G>A，c.11917 C>T，c.11946 C>T，c.13405 C>A，c.13429 T>C，c.13438 T>C，c.14108 A>C，c.14516 A>G，c.14522 G>A，c.14654 C>T，c.15834 G>A，c.15873 G>A，c.17560 C>G and c.17770 G>A，were found
within the exon region of NHE1 gene in yellow broilers，these SNPs were synonymous with cSNP and the changes of
amino acid were not caused by further analysis. In addition，SNPs and insertion/deletion mutations were also found
within the intron region of NHE1 gene in yellow broilers，the AGGGCTGC was inserted between c.12239 and 12240
and the TGAAGCC was inserted between c.13114 and 13115 within the intron 2 of NHE1 gene，respectively，and the TGTCCTGTGTCCTCCCTGC among c.17073-17091 was deleted in intron 11 of NHE1 gene. Furthermore，6 SNPs
c.309 G>C，c.11653 C>T，c.13405 C>A，c.13438 T>C，c.14108 A>C and c.14516 A>G within the exon region，
as well as insertion/deletion mutations within the intron region that were same with yellow broilers，were found in
NHE1 gene of the negative and positive chickens after inoculated by ALV-J. The results demonstrated that the genetic
mutation loci found in receptor gene NHE1 may not be used as potential genetic resistance loci of ALV-J. |
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