周述波1,贺立红2,贺立静1,林 伟1.ABA 抑制杂交水稻亲本种子萌发的基础生理变化研究[J].广东农业科学,2016,43(8):1-5 |
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ABA 抑制杂交水稻亲本种子萌发的基础生理变化研究 |
Study on basic physiological effects of exogenous ABA on germination of hybrid rice parent line seeds |
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DOI:10.16768/j.issn.1004-874X.2016.08.001 |
中文关键词: ABA 杂交水稻 抑制 萌发 |
英文关键词: abscisic acid hybrid rice inhibition seed germination |
基金项目:海南省自然科学基金(20153150);三亚市院地科技合作项目(2014YD32);琼州学院校级青年科学基金(QYQN201243) |
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中文摘要: |
用外源ABA 对杂交水稻亲本金23B 和V20B 种子进行处理,其萌发过程中生理变化结果表明,ABA 浓度为70~110 mg/L 时,随着浓度增加,对两个水稻品种亲本种子的萌发抑制效果显著;ABA 浓度为110 mg/L 时,降低了两品种种子萌发过程中可溶性糖的含量、α- 淀粉酶的活性,改变了可溶性蛋白的时空规律,降低了种子内源GA3 与IAA 的含量,增加了内源ABA 含量;种子萌发受到抑制不是由种子内源ABA 含量的高低决定,用外源ABA 处理种子,金23B 内源ABA 含量高于V20B,但对种子萌发抑制的效果前者差于后者,种子萌发抑制与GA3 /ABA 比值大小密切相关。 |
英文摘要: |
With seeds of hybrid rice parent line Jin 23B and V20B as experimental materials,the study was focused on the physiological effects of exogenous ABA on germination of hybrid rice seeds. The results showed that seed germination was significantly restrained by 70-110 mg/L ABA with the content increasing. Physiological and biochemical analysis indicated that α-amylases activity degraded,soluble sugar content reduced,soluble protein content changed,and phytohormones contents were affected,endogenous GA3 and IAA degraded,and endogenous ABA content increased by 110 mg/L ABA. The content of endogenous ABA of Jin23B was higher than that of V20B,but the inhibition effect in seed germination of Jin23B was worse than that of V20B,therefore,seed germination was not determined by the level of endogenous ABA content in seed,and was closely related to the GA3 /ABA ratio. |
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