| 马 壮1，2，彭新宇2，叶得河1，高 彪2，袁明贵2，尹烨华2，徐志宏2.冷鲜鸡产气荚膜梭菌及其毒素的潜在危害分析[J].广东农业科学,2016,43(8):113-118 |
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冷鲜鸡产气荚膜梭菌及其毒素的潜在危害分析 |
| Potential hazards analysis of Clostridium perfringens and its toxins from cold fresh chicken |
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| DOI：10.16768/j.issn.1004-874X.2016.08.019 |
| 中文关键词: 冷鲜鸡 产气荚膜梭菌 毒素 耐药性 Eric-PCR |
| 英文关键词: Chicken Clostridium perfringens toxin drug sensitivity test Eric-PCR |
| 基金项目:国家公益性行业（农业）科研专项（201303040-16）；广东省科学技术厅省级科研计划（2013B060400037，2014A020208133）; 广东省科学技术厅国际合作基地项目（2015B050501007) |
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| 中文摘要: |
| 为调查广州市冷鲜鸡产气荚膜梭菌及其毒素危害，从广州市各大型超市随机采集鸡肉样品进行产气荚膜梭菌分离、鉴定和毒素分型，同时检测分离菌株对常用抗生素的抗药性，并采用Eric-PCR 对分离出梭菌进行亚型分析。结果表明，从78 个样本中分离出57 株产气荚膜梭菌，所有菌株均为A 型，其中，cpb2（Atypical）型40 株，cpb2（consensus）型1 株；含netB 基因菌株1 株；药敏结果显示，分离出的产气荚膜梭菌大部分对氟苯尼考、氨苄西林、青霉素敏感；值得注意的是产气荚膜梭菌分离株对仅可用于人的抗生素克林霉素、头孢氨卞的耐药率分别达73.68% 和31.58%。Eric-PCR 结果显示，相似性为80% 时，57株梭菌可分为13 个亚型，提示污染来源的多样性。 |
| 英文摘要: |
| Retail cold fresh chicken samples were collected randomly from several Guangzhou supermarkets to investigate the hazards of Clostridium perfringens and its toxins from the chicken. Results showed that 57 isolates were isolated from 78 chicken samples，all isolates were C. perfringens type A. One of the isolates carried netB gene，40 isolates carried cpb2 Atypical gene，one isolate carried cpb2 consensus gene. Results of drug resistance test showed that most of the isolates were sensitive to florfenicol，ampicillin and penicillin. It was noteworthy that some isolates were resistant to clindamycin（73.68%） and cefalexin（31.58%），which could only be used in human being.Results of Eric-PCR showed that when similarity was 80%，these isolates could be classified into 13 subtypes，that implied the diversity of pollution sources cold fresh. |
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