| 张爱玲1，2，孙显月2，尹 琪3，李加琪2，张 豪2.猪羰基还原酶1（CBR1）基因克隆及组织表达谱分析[J].广东农业科学,2016,43(8):151-157 |
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猪羰基还原酶1（CBR1）基因克隆及组织表达谱分析 |
| Cloning and expression of porcine carbonyl reductase1（CBR1） gene |
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| DOI：10.16768/j.issn.1004-874X.2016.08.025 |
| 中文关键词: 猪 CBR1 基因表达 启动子 |
| 英文关键词: pig CBR1 gene expression promoter |
| 基金项目:国家自然科学基金（31201771）；国家科技支撑计划项目（2011BAD28B01）；国家现代农业产业技术体系专项（CARS-36） |
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| 中文摘要: |
| 为了深入研究猪羰基还原酶1（CBR1）基因的表达调控，通过PCR 获得包括猪CBR1 基因启动子和外显子、内含子的序列，并进行蛋白结构分析和系统进化关系比较，通过RT-PCR 方法检测了CBR1基因在不同组织的表达丰度。结果表明，猪CBR1 基因启动子区长度为2 875 bp，具有潜在典型的NFκB 等转录因子结合位点；CBR1 基因由3 个外显子和2 个内含子组成，外显子长度分别为289、108 和437 bp，其中5′UTR 和3′UTR 分别为107 bp 和242 bp，内含子长度分别为572 bp 和3 219 bp，编码区由289 个氨基酸组成；该蛋白为亲水性蛋白，无明显的信号肽，有2 个跨膜区域；蛋白二级结构和三级结构主要由7个α- 螺旋、7 个β- 折叠以及loop 环构成。猪CBR1 基因氨基酸序列与人、绵羊氨基酸的相似性较高，为84.48%。qRT-PCR 结果表明，怀孕12 d 的二花脸子宫内膜中CBR1 基因mRNA 的表达量极显著高于长大二元母猪；在怀孕12 d 母猪9 个组织中，CBR1 在肾脏中的表达量最高，其次是肝脏和小肠 。 |
| 英文摘要: |
| The promoter，exons and introns of porcine carbonyl reductase1 gene（ CBR1） were cloned，and the structure of CBR1 protein was analyzed. The similarity of amino acid was compared with other species and the phylogen of the gene was constructed. The tissues expression of the gene was detected by RT-PCR. The results showed that the promoter of the gene contained 2 875 bp，and typical binding sites of NFκB were predicted. The gene included three exons and two introns，and the formers spanned 289，108 and 437 bp，respectively. The two introns spanned 572 bp and 3 219 bp，respectively. The 5′UTR of 107 bp and 3′UTR of 242 bp were identified.The CDS was encoded by 289 amino acids. The CBR1 protein was hydrophilic，and contained two transmembrane domains but no signal peptide was found. The tertiary structure of the protein was formed by seven α-helixes，seven sheets，and loops. The amino acid sequence of the gene exhibited higher similarity of 84.48% with that of human and sheep. The CBR1 mRNA in the endometrium of Erhualian sows was higher significantly than that of Landrace × large white sows on GD12（ gestation day 12）（ P<0.01）. The gene was expressed with the highest level in kidney tissue and the higher in liver and small bowel tissues of Erhualian on GD12. |
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