| 张雪莲1，黄雪梅2，张 灿2，张昭其2，庞学群1.INA 抑制香蕉果实中活性氧积累和衰老进程提高果实的抗病性[J].广东农业科学,2016,43(11):109-115 |
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INA 抑制香蕉果实中活性氧积累和衰老进程提高果实的抗病性 |
| 2，6-Dichloroisonicotianic acid repressed reactive oxygen species content and fruit ripening to induce diseasetolerance in banana（ Musa AAA. cv. Dongguang） |
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| DOI：10.16768/j.issn.1004-874X.2016.11.017 |
| 中文关键词: INA 处理 抗病性 活性氧 APX P450 |
| 英文关键词: INA treatment disease tolerance reactive oxygen species（ ROS） APX P450 |
| 基金项目:国家“十二五”科技支撑计划项目（2015BAD16B07）；国家自然科学基金（30771515） |
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| 摘要点击次数: 1951 |
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| 中文摘要: |
| 探讨了香蕉贮藏期间INA 处理对广东果实抗病性的影响及影响机制。结果表明：香蕉果实在
INA 处理后接种炭疽病菌孢子，贮藏15 d 后，INA 处理的果实病斑直径比对照果实明显减小，INA 处理的
MDA 含量显著低于对照，表明INA 处理能够延缓果实的衰老；INA 处理果实的H2O2 含量从贮藏8 d 开始明
显低于对照，而NADPH 氧化酶活性则在贮藏10 d 开始高于对照；在整个贮藏期间，INA 处理的CAT 活性
均明显低于对照，抗坏血酸氧化酶活性则明显高于对照。在贮藏后期，INA 促进APX 基因的表达，却抑制了
CAT 和后熟相关基因P450 的表达上调。说明在贮藏期间INA 主要通过促进防御酶APX 基因和酶活力抑制
后期果实活性氧含量的增加，以诱导香蕉果实抗病性的增加。 |
| 英文摘要: |
| The possible mechanism of INA on disease-tolerance in postharvested banana （Musa AAA. cv.
Dongguang） was investgated. Ther results showed that INA treatment effectively reduced the disease spot sizes on the
peel of banana fruit when inoculated with spore suspension of Collectotrichum musae after INA treatment and cultured
for 15 d. During storage，MDA content increased in both INA treatment and control；however，INA treatment
markedly inhibited the MDA content，suggesting that INA retarded the fruit ripening. The content of H2O2 in INA
treatment was obviously lower than that in control from the 8th d，while high activety of NADPH oxidase in INA
treatment was found from the 10th d. An induced CAT activity was found in control during storage；however，high
APX activity was detected in INA treatment. During the later storage，INA induced the APX gene expression，but
repressed the expression of CAT and P450. The results suggested that enhancing APX activity/gene expression could
reduce H2O2 level to improve banana disease tolerance during storage. |
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