| 李智丽1，黄淑坚1，马静云2，毕英佐2.2011—2015 年我国南方地区PEDV 分子流行病学调查及S 基因遗传变异分析[J].广东农业科学,2016,43(11):127-135 |
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2011—2015 年我国南方地区PEDV 分子流行病学调查及S 基因遗传变异分析 |
| Molecular epidemiological investigation of PEDV and analysis of genetic variation of S gene in South Chinaduring 2011-2015 |
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| DOI：10.16768/j.issn.1004-874X.2016.11.020 |
| 中文关键词: 猪流行性腹泻病毒 动态监控 S 基因 遗传变异 |
| 英文关键词: porcine epidemic diarrhea virus dynamic investigation Spike gene genetic variation |
| 基金项目:国家自然科学基金（31502071） |
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| 摘要点击次数: 2216 |
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| 中文摘要: |
| 应用RT-PCR 方法对南方地区五省（广东、广西、福建、四川、江苏）18 个猪场7 日龄以内发
病仔猪的小肠组织和粪便样本进行猪流行性腹泻病毒（PEDV）检测，对9 个猪场进行为期5 年的动态监
控，并对2011—2015 年期间分离的35 个PEDV 流行株的S 基因进行测序，与GenBank 中的PEDV 参考序
列进行比较，并绘制了系统进化树。结果显示，上述地区五省存在Group1 和 Group 2 两种类型毒株。Group1
与美国、韩国毒株较为接近，可分为G1-1、G1-2、G1-3 和G1-4 等4 个亚群，Group 2 与2004 年国内分离
毒株较为接近，可分为G2-1、G2-2 两个亚群。25 个中国南方地区毒株集中于 G1-2 和 G1-3，而另外8 株
与2004 年国内毒株（JS-2004-2）同属G2-2 亚群。不同类型的毒株引发的PEDV 临床表现及死亡率不
同，G2-2 亚群的毒株引发的PED 相对缓和，死亡率较低且病程较短，而位于G1-2 和G1-3 亚群（尤其是
G1-3）的毒株则引起大规模急性暴发，病程较长，死亡率较高。 |
| 英文摘要: |
| The study conducted a large scale molecular epidemiological investigation and five years monitoring
of nine swine herds with the outbreak of diarrhea located in geographically separate regions of south China by
RT-PCR. Porcine intestinal and fecal samples were collected from 18 swine farms in five provinces（ Guangdong
province，Guangxi province，Fujian province，Sichuan province，Jiangsu province） in south China with the
piglets younger than 7 days old that showed watery diarrhea and dehydration. 35 spike（ S） genes were determined
to analyze the genetic diversity and epidemiological features during 2011-2015. The results showed that all PEDV
strains could be divided into two groups，Group1 and Group 2. Group1 had a close relationship with the United
States strains and Taiwan strains，and had four subgroups，G1-1，G1-2，G1-3 and G1-4. Group 2 felled into the
same branch with the previous Chinese isolates from 2004 that had two subgroups，G2-1 and G2-2. The subgroup
G1-2 and G1-3 comprised most of the strains isolated in south China while the subgroup G2-2 comprised the strain isolated in 2004（ JS-2004-2） and eight else isolated in south China in our study. It is remarkably that
clinical morbidity caused by two types of PEDV strains were quite different，strains in G2-2 caused relatively
minor mild clinical manifestations，presenting a smaller mortality and shorter onset period，while strains in
G1-2 and G1-3 is caused an outbreak of acute infectious diseases，showing a larger mortality and longer period
especially PEDV strains in G1-3. |
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