宋 顺1,2,黄东梅1,2,徐碧玉3,金志强1,2,孙 威1,2,
许桂莺1,2,李敬阳1,2,许 奕1,2,3.非生物胁迫对水通道蛋白启动子活性的调节作用[J].广东农业科学,2016,43(12):1-5 |
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非生物胁迫对水通道蛋白启动子活性的调节作用 |
Regulation of abiotic stress on aquaporin promoter activity |
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DOI:10.16768/j.issn.1004-874X.2016.12.001 |
中文关键词: 水通道蛋白 启动子 逆境 GUS |
英文关键词: aquaporin promoter stress GUS |
基金项目:国家自然科学基金(31501371) |
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中文摘要: |
前期通过染色体步移法克隆得到巴西蕉水通道蛋白基因MaPIP1-2 上游841 bp 的启动子序
列,分析其启动子序列含有响应干旱和高盐等非生物胁迫的顺式作用元件。为了进一步研究该启动子的功
能,成功构建MaPIP1-2 基因启动子的植物表达载体,并且转化拟南芥,在MaPIP1-2::GUS 转基因拟南芥
中发现,MaPIP1-2 启动子活性受到干旱和高盐的诱导表达,随着浓度增加,其启动活性增强。 |
英文摘要: |
A 841bp sequence of MaPIP1-2 promoter was isolated from banana( Musa acuminat L. AAA
group cv. Brazilian) through chromosome walking PCR. PLACE analysis indicated that MaPIP1-2 promoter
contatined abiotic stress-responsive elements,such as drought-responsive elements and salt-related cis-elements.
To investigate the function of the promoter,the plant expression vector with MaPIP1-2 promoter was successfully
constructed and transformed into Arabidopsis thaliana. In MaPIP1-2::GUS-expressing Arabidopsis,the activity
of MaPIP1-2 promoter was found to be associated with the drought and salt treatment. The activity of MaPIP1-2
promoter was enhanced with the increasing concentration of salt and mannitol. |
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