| 汤鸣强，陈淑贤.三唑磷农药降解酶的定位及酶学性质研究[J].广东农业科学,2017,44(1):115-121 |
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三唑磷农药降解酶的定位及酶学性质研究 |
| Distribution and properties of triazophos-degrading enzyme |
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| DOI：10.16768/j.issn.1004-874X.2017.01.018 |
| 中文关键词: 三唑磷降解菌TAP-1 降解酶 酶定位 酶学特性 |
| 英文关键词: triazophos-degrading bacteria TAP-1 degrading enzyme enzyme localization enzymatic
property |
| 基金项目:福建省自然科学基金（2012J01118） |
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| 摘要点击次数: 3377 |
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| 中文摘要: |
| 采用超声波破碎法破碎三唑磷农药降解菌芽孢杆菌TAP-1，研究不同细胞组分对三唑磷农药
的降解率，应用液相色谱法研究三唑磷农药降解酶的酶学性质。结果表明，胞外酶、胞间粗酶液和胞内酶对
三唑磷的降解率分别为2.8（±0.34）%、12.8（±2.44）% 和84.4（±6.71）%。菌株TAP-1 降解三唑磷农
药的主要活性物质位于降解菌细胞内，属于胞内酶组分。该酶最适作用pH 值为6.5～7.0，在pH 5.0～7.5 之
间，酶活力均能保持在最高酶活力的68% 以上。最适反应温度为30℃，在25～40℃温度范围内能保持75%
以上的降解活性。不同化学试剂和金属离子对酶活性产生不同的影响。巯基修饰剂、EDTA、Hg2+ 和Mn2+ 对
酶活性有抑制作用。Mg2+、Co2+ 和Fe3+ 对酶活性有促进作用。该降解酶米氏常数（Km）为23.02 μmol/L，最
大反应速度（Vmax）为 0.738 μmol/mg·min。 |
| 英文摘要: |
| To investigate the effects of different fractions of bacterial strain Bacillus TAP-1 on the degradation
rate of tirazophos, sonication method was applied to lyse the TAP-1 cells to obtain triazophos-degrading enzyme，
while HPLC（High Performance Liquid Chromatography） method was used to determine the enzymatic properties.
The results showed that the degradation rate of triazophos by intracellular, intercellular and extracellular extracts
were 84.4（±6.71）%, 12.8（±2.44）%, and 2.8（±0.34）%, respectively. The active substances of
triazophos-degrading were intracellular fractions. The enzyme demonstrated greatest enzymatic activity in the pH
range of 6.5-7.0 with its highest activity occurring in the pH range of 5.0-7.5. Enzymatic activity occurred at an
optimum temperature of 30℃ for the degradation of triazophos；activity remained above 75% of the maximum over
a temperature range of 25℃-40℃. Different chemicals and metallic ions also had different effects on its activity.
Its activity can be strongly inhibited by thiol modifier, ETDA, Hg2+ and Mn2+, but stimulated by Mg2+，Co2+ and
Fe3+. The Km value and the maximal degradation rate of triazophos-hydrolase were 23.02 μmol/L and 0.738
μmol/mg·min, respectively. |
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